AB323289

Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal alpha 1 Antitrypsin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

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Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (AB323289)

sELISA

Supplier Data

Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (AB323289)

Sandwich ELISA of ab323286 using Human SERPINA1 immunogen protein standard curve with the capture antibody ab323288 at 2 µg/mL and detector antibody ab323289 dilution at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown).

sELISA

Supplier Data

Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (AB323289)

Interpolated concentration of SERPINA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1/10, 000 dilution Human Plasma and Human Serum. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). We do not guarantee same sensitivity level due to biological variation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR26599-43

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab323289 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human alpha 1 Antitrypsin. The recommended pair for sandwich ELISA is:
Capture: ab323288, Human alpha 1 Antitrypsin Capture Antibody (unconjugated)
Detector: ab323289, Human alpha 1 Antitrypsin Detector Antibody (unconjugated)
The reference range value is 97-12500 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector)