Anti-alpha 1 Antitrypsin抗体(ab9373)
Key features and details
- Rabbit polyclonal to alpha 1 Antitrypsin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-alpha 1 Antitrypsin抗体
参阅全部 alpha 1 Antitrypsin 一抗 -
描述
兔多克隆抗体to alpha 1 Antitrypsin -
宿主
Rabbit -
特异性
The antibody is specific against human alpha-1-antitrypsin. -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mammals
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免疫原
Full length native protein (purified) corresponding to Human alpha 1 Antitrypsin. Isolated from human serum.
Database link: P01009 -
阳性对照
- IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-P
Use a concentration of 1 µg/ml. |
靶标
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功能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
组织特异性
Ubiquitous. Expressed in leukocytes and plasma. -
疾病相关
Alpha-1-antitrypsin deficiency -
序列相似性
Belongs to the serpin family. -
结构域
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻译后修饰
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
细胞定位
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
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别名
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha 1 Antitrypsin with ab9373.
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Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (ab9373)ICC/IF image of ab9373 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9373, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (ab9373)ab9373 staining alpha 1 Antitrypsin in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab9373 被引用在 12 文献中.
- Colasanti T & Conti F Autoantibody Recognition of Natural and Homocysteinylated Alpha-1 Antitrypsin: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Quantification in Sera. Methods Mol Biol 2750:135-141 (2024). PubMed: 38108974
- Hendriks D et al. Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver. Nat Protoc 16:182-217 (2021). PubMed: 33247284
- Lee JH et al. Human Liver Stem Cell Transplantation Alleviates Liver Fibrosis in a Rat Model of CCl4-Induced Liver Fibrosis. Int J Stem Cells 14:475-484 (2021). PubMed: 34711695
- Smith SM & Melrose J A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-a-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface. Int J Mol Sci 20:N/A (2019). PubMed: 30678366
- Skurikhin EG et al. Endothelial Progenitor Cells as Pathogenetic and Diagnostic Factors, and Potential Targets for GLP-1 in Combination with Metabolic Syndrome and Chronic Obstructive Pulmonary Disease. Int J Mol Sci 20:N/A (2019). PubMed: 30836679