Anti-ALP抗体(ab224335)
Key features and details
- Rabbit polyclonal to ALP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ALP抗体
参阅全部 ALP 一抗 -
描述
兔多克隆抗体to ALP -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig
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免疫原
Recombinant fragment corresponding to Human ALP aa 1-100.
Database link: Q53GG5 -
阳性对照
- IHC-P: Human skeletal muscle tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224335于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in the organization of actin filament arrays within muscle cells. -
组织特异性
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific. -
序列相似性
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
细胞定位
Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines. - Information by UniProt
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数据库链接
- Entrez Gene: 538516 Cow
- Entrez Gene: 27295 Human
- Entrez Gene: 53318 Mouse
- Entrez Gene: 414421 Pig
- Entrez Gene: 114108 Rat
- Omim: 605889 Human
- SwissProt: Q3SYZ8 Cow
- SwissProt: Q53GG5 Human
see all -
别名
- Actinin associated LIM protein antibody
- Actinin-associated LIM protein antibody
- Alpha actinin 2 associated LIM protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody (ab224335)
Paraffin-embedded human skeletal muscle tissue stained for ALP using ab224335 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ALP antibody (ab224335)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ALP (green) using ab224335 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab224335 被引用在 19 文献中.
- Sun B et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
- Zhang H et al. Reprogramming mitochondrial metabolism of macrophages by miRNA-released microporous coatings to prevent peri-implantitis. J Nanobiotechnology 21:485 (2023). PubMed: 38105216
- Zhang Y et al. Fabrication of Interleukin-4 Encapsulated Bioactive Microdroplets for Regulating Inflammation and Promoting Osteogenesis. Int J Nanomedicine 18:2019-2035 (2023). PubMed: 37155503
- Li X et al. FRZB affects Staphylococcus aureus‑induced osteomyelitis in human bone marrow derived stem cells by regulating the Wnt/β‑catenin signaling pathway. Exp Ther Med 26:531 (2023). PubMed: 37869648
- Kızıldağa A et al. Therapeutic effects of diosgenin on alveolar bone loss and apoptosis in diabetic rats with experimental periodontitis. Iran J Basic Med Sci 26:785-790 (2023). PubMed: 37396943