Anti-ALKBH8抗体(ab113512)
Key features and details
- Rabbit polyclonal to ALKBH8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ALKBH8抗体 -
描述
兔多克隆抗体to ALKBH8 -
宿主
Rabbit -
特异性
ab113512 is also predicted to react with isoform 4 of Human ALKBH8 (76kDa). -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to N terminal amino acids 2-238 of Human ALKBH8 (BC015183).
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阳性对照
- Human fetal spleen and A549 cell lysates; Human fetal spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab113512 is purified by a protein affinity column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 75 kDa.
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| IHC-P |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 75 kDa. |
|
IHC-P
1/100 - 1/500. |
靶标
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功能
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. -
组织特异性
Widely expressed, with highest expression in spleen, followed by pancreas and lung. -
序列相似性
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 RRM (RNA recognition motif) domain. -
细胞定位
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 91801 Human
- Entrez Gene: 67667 Mouse
- Entrez Gene: 366783 Rat
- Omim: 613306 Human
- SwissProt: Q96BT7 Human
- SwissProt: Q80Y20 Mouse
- Unigene: 503763 Human
- Unigene: 116968 Mouse
see all -
别名
- 4930562C03Rik antibody
- 8030431D03Rik antibody
- 9430088N01Rik antibody
see all
图片
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Western blot - Anti-ALKBH8 antibody (ab113512)All lanes : Anti-ALKBH8 antibody (ab113512) at 1/500 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : A549 cell lysate
Predicted band size: 75 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH8 antibody (ab113512)ab113512 at 1/100 dilution staining ALKBH8 in Formalin-fixed, Paraffin-embedded Human fetal spleen tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab113512 被引用在 1 文献中.
- Delaunay S et al. Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer. J Exp Med 213:2503-2523 (2016). PubMed: 27811057