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重组RabMAb

重组Anti-ALKBH4抗体[EPR19216] - BSA and Azide free (ab251206)

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Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
  • Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
  • Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
  • Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19216] to ALKBH4 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-ALKBH4抗体[EPR19216] - BSA and Azide free
    参阅全部 ALKBH4 一抗

  • 描述

    兔单克隆抗体[EPR19216] to ALKBH4 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Non human primates

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    ab251206 is the carrier-free version of ab195379.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab251206于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation.

  • 组织特异性

    Widely expressed, with highest expression in pancreas, ovary and spleen.

  • 序列相似性

    Belongs to the alkB family.

  • 细胞定位

    Cytoplasm. Nucleus. Midbody. Associates with the contractile ring and midbody.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 2010004B12Rik antibody
      • ABH4 antibody
      • Abj4 antibody
      • AlkB, alkylation repair homolog 4 (E. coli) antibody
      • ALKB4_HUMAN antibody
      • Alkbh4 antibody
      • Alkylated DNA repair protein alkB homolog 4 antibody
      • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4 antibody
      • FLJ20013 antibody
      • Probable alpha-ketoglutarate dependent dioxygenase ABH4 antibody
      • RGD1308608 antibody
      see all

    图片

    • Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
      Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
      All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 33 kDa
      Observed band size: 33 kDa


      Exposure time: 1 minute


      This data was developed using ab195379, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
      Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
      All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
      Lane 4 : Human pancreas tissue lysate
      Lane 5 : WI-38 (human fetal lung fibroblast cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 33 kDa
      Observed band size: 33 kDa


      Exposure time: 1 minute


      This data was developed using ab195379, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
      Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
      This data was developed using ab195379, the same antibody clone in a different buffer formulation.ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
      Lane 1: HEK-293 whole cell lysate 10ug (Input).
      Lane 2: ab195379 IP in HEK-293 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 5 seconds.
    • Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
      Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab251206？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab251206 尚未被引用在任何文献中。

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