Anti-ALKBH4抗体(ab113500)
Key features and details
- Rabbit polyclonal to ALKBH4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ALKBH4抗体
参阅全部 ALKBH4 一抗 -
描述
兔多克隆抗体to ALKBH4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to amino acids 38-282 of Human ALKBH4 (BC002820).
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阳性对照
- Human fetal spleen, fetal liver and fetal kidney lysates; Human fetal pancreas tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113500于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa).
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| IHC-P |
1/100 - 1/500.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa). |
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IHC-P
1/100 - 1/500. |
靶标
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功能
Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation. -
组织特异性
Widely expressed, with highest expression in pancreas, ovary and spleen. -
序列相似性
Belongs to the alkB family. -
细胞定位
Cytoplasm. Nucleus. Midbody. Associates with the contractile ring and midbody. - Information by UniProt
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数据库链接
- Entrez Gene: 54784 Human
- Entrez Gene: 72041 Mouse
- Entrez Gene: 288587 Rat
- Omim: 613302 Human
- SwissProt: Q9NXW9 Human
- SwissProt: Q9D8F1 Mouse
- Unigene: 658598 Human
- Unigene: 486605 Mouse
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别名
- 2010004B12Rik antibody
- ABH4 antibody
- Abj4 antibody
see all
图片
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Western blot - Anti-ALKBH4 antibody (ab113500)All lanes : Anti-ALKBH4 antibody (ab113500) at 1/500 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal kidney lysate
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH4 antibody (ab113500)ab113500, at 1/100 dilution, staining ALKBH4 in Formalin-fixed, Paraffin-embedded Human fetal pancreas tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113500 尚未被引用在任何文献中。