Alkaline Phosphatase Anti-Digoxigenin抗体[21H8] (ab119345)
Key features and details
- Alkaline Phosphatase Mouse monoclonal [21H8] to Digoxigenin
- Suitable for: WB, ELISA, IHC-Fr, Southern Blot, ICC/IF
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Alkaline Phosphatase Anti-Digoxigenin抗体[21H8]
参阅全部 Digoxigenin 一抗 -
描述
Alkaline Phosphatase小鼠单克隆抗体[21H8] to Digoxigenin -
宿主
Mouse -
偶联物
Alkaline Phosphatase -
特异性
ab119345 reacts with free and bound digoxigenin. It is useful for detection of digoxigenin labelled compounds. -
经测试应用
适用于: WB, ELISA, IHC-Fr, Southern Blot, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Digoxigenin
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 50% Glycerol, 0.121% Tris, 0.81% Sodium chloride, 0.01% Magnesium chloride, 0.014% Zinc chloride -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
21H8 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119345于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000.
|
|
| ELISA |
1/500 - 1/5000.
|
|
| IHC-Fr |
1/200 - 1/2000.
|
|
| Southern Blot |
1/500 - 1/5000.
|
|
| ICC/IF |
1/200 - 1/2000.
|
| 说明 |
|---|
|
WB
1/500 - 1/5000. |
|
ELISA
1/500 - 1/5000. |
|
IHC-Fr
1/200 - 1/2000. |
|
Southern Blot
1/500 - 1/5000. |
|
ICC/IF
1/200 - 1/2000. |
靶标
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相关性
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
别名
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab119345 被引用在 8 文献中.
- Wu Y et al. The Role of Sorting Nexin 17 in Cardiac Development. Front Cardiovasc Med 8:748891 (2021). PubMed: 34988124
- Abo-Ahmed AI & Emam MA Expression of vesicular glutamate transporter 2 and 3 and glutamate receptor 1 and 2 mRNAs in the retina of adult laughing doves (Streptopelia senegalensis): An in situ hybridization study. Acta Histochem 122:151597 (2020). PubMed: 32778249
- Zhang H et al. Serum exosomes mediate delivery of arginase 1 as a novel mechanism for endothelial dysfunction in diabetes. Proc Natl Acad Sci U S A 115:E6927-E6936 (2018). PubMed: 29967177
- Liu Y et al. Cystic fibrosis transmembrane conductance regulator mediates tenogenic differentiation of tendon-derived stem cells and tendon repair: accelerating tendon injury healing by intervening in its downstream signaling. FASEB J 31:3800-3815 (2017). PubMed: 28495756
- Ohana G et al. A3 adenosine receptor agonist, CF102, protects against hepatic ischemia/reperfusion injury following partial hepatectomy. Mol Med Rep 14:4335-4341 (2016). PubMed: 27666664
- Liang DY et al. Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis. Int J Mol Med 38:131-8 (2016). IHC ; Human . PubMed: 27246196
- Li DQ et al. Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma. Oncol Lett 11:3003-3008 (2016). PubMed: 27123053
- Parry L et al. Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models. J Vis Exp N/A:N/A (2015). PubMed: 26649885