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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases

Anti-Alkaline Phosphatase抗体(ab354)

  • Datasheet
  • SDS
Reviews (2)Q&A (7)References (8)

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Key features and details

  • Rabbit polyclonal to Alkaline Phosphatase
  • Suitable for: ELISA, IP, WB
  • Reacts with: Escherichia coli
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Alkaline Phosphatase抗体
    参阅全部 Alkaline Phosphatase 一抗
  • 描述

    兔多克隆抗体to Alkaline Phosphatase
  • 宿主

    Rabbit
  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
  • 经测试应用

    适用于: ELISA, IP, WBmore details
  • 种属反应性

    与反应: Escherichia coli
    不与反应: Mammals
  • 免疫原

    Full length protein corresponding to Alkaline Phosphatase. Full length protein (E. coli).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 纯化说明

    This product was prepared from monospecific antiserum by a delipidation and defibrination.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Alkaline Phosphatase
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Phosphatases

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Alkaline Phosphatase protein (ab157880)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab354于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 序列相似性

    Belongs to the alkaline phosphatase family.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P09923 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 别名

    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

实验方案

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (8)

发表研究结果有使用 ab354?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab354 被引用在 8 文献中.

  • Hodgkinson T  et al. The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells. Sci Adv 7:N/A (2021). PubMed: 33637520
  • Hemnes AR  et al. BMPR2 dysfunction impairs insulin signaling and glucose homeostasis in cardiomyocytes. Am J Physiol Lung Cell Mol Physiol 318:L429-L441 (2020). PubMed: 31850803
  • Asgharian-Rezaee M  et al. Comparison of Osteogenic Potential of Phenytoin with Dexamethasone in Cultured Dental Pulp Stem Cells. Rep Biochem Mol Biol 9:331-337 (2020). PubMed: 33649727
  • Wang C  et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018). PubMed: 30043216
  • Tsimbouri PM  et al. Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nat Biomed Eng 1:758-770 (2017). PubMed: 31015671
  • Bross P  et al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). WB ; Escherichia coli . PubMed: 18400758
  • Imperi F  et al. Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154:2804-13 (2008). PubMed: 18757814
  • Downer R  et al. The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall-associated protein. J Biol Chem 277:243-50 (2002). PubMed: 11684686

客户评价及客户问答

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1-9 of 9 Abreviews or Q&A

ELISA abreview for Anti-Alkaline Phosphatase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Escherichia coli Recombinant protein (Mach1)
Specification
Mach1
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type
Indirect
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 26 2010

Western blot abreview for Anti-Alkaline Phosphatase antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (Mach1)
Loading amount
0.5 µg
Specification
Mach1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 19 2010

Question

Please help with the following questions: What is the concentration of the antibody to bacterial alkaline phosphatase? Is this an affinity purified polyclonal? Are there ANY other proteins in the preparation?

Read More

Abcam community

Verified customer

Asked on Jun 17 2005

Answer

Thank you for your enquiry. This product is sold as tissue culture supernatant and not affinity purified. As a tissue culture supernatant this product will contain additional proteins and the IgG concentration has not been determined. Typically IgG concentrations in tissue culture supernatant will range between 0.5 and 1 mg/ml. If you have any further questions then please get back in touch with us.

Read More

Abcam Scientific Support

回复于 Jun 21 2005

Question

I saw an enquiry dated 7-Feb-2001 asking whether this antibody can detect secreted alk phos (SEAP). At the time, the answer was that you have no reports -- have you heard anything further about this application since?

Read More

Abcam community

Verified customer

Asked on Sep 04 2002

Answer

This antibody should detect SEAP from E.coli. It has not been tested for cross-reactivity in other bacteria and does not cross-react with AP from mammalian species.

Read More

Abcam Scientific Support

回复于 Sep 05 2002

Question

I'm having trouble using this product for ELISA at 2µg/ml. Please let me know the recommended conditions.

Read More

Abcam community

Verified customer

Asked on Nov 01 2001

Answer

A working dilution from our test using lot 4679(assayed againest 1.0 ug of Alkaline Phosphatase in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anit- Rabbit IgG, was 1:8,000 to 1:40,000 (volume:volume).

Read More

Danielle Miller

Abcam Scientific Support

回复于 Nov 14 2001

Question

We want to use alkaline phosphatase antibody to determine ALP releasing in periodontal cells (Osteoblasts, PDL fibroblasts and cementoblasts). Does Alp1-ab354 test ALP expression in bone-like cells by immunohistochemistry?

Read More

Abcam community

Verified customer

Asked on Oct 31 2001

Answer

No this antibody is not suitable as it does not cross react with the mammalian form of Alkaline Phosphatase. The immunogen is Alkaline Phosphatase from E.coli. We suggest you review our other alkaline phosphatase antibodies, many of which have been tested in IHC.

Read More

Abcam Scientific Support

回复于 Nov 01 2001

Question

I would like to know whether your alkaline phosphatase antibody is a marker of Plasma membrane or not.

Read More

Abcam community

Verified customer

Asked on Mar 06 2001

Answer

No, this antibody is not a marker of plasma membrane.

Read More

Abcam Scientific Support

回复于 Mar 07 2001

Question

Do you know if your Alp1 Ab can detect secreted alkaline phosphatase (SEAP)?

Read More

Abcam community

Verified customer

Asked on Feb 06 2001

Answer

We have no reports of it being used in this application, but do not see a reason why it should not recognise SEAP.

Read More

Abcam Scientific Support

回复于 Feb 07 2001

Question

We want to use alkaline phosphatse as a marker in E. coli cell fraction experiments. To monitor alk. phosphatase we want to use your antibody. Do you know if alk. phosphatase is expressed under all condition in E. coli (at different levels). Many thanks! Jan-Willem

Read More

Abcam community

Verified customer

Asked on Jan 18 2001

Answer

We believe it is although it might be more highly expressed in stationary phase and be strain dependent. Abcam

Read More

Abcam Scientific Support

回复于 Jan 18 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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