Alkaline Phosphatase Anti-6X His tag®抗体[HIS-1] (ab49746)
Key features and details
- Alkaline Phosphatase Mouse monoclonal [HIS-1] to 6X His tag®
- Suitable for: WB, Dot blot, ELISA
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Alkaline Phosphatase Anti-6X His tag®抗体[HIS-1]
参阅全部 6X His tag® 一抗 -
描述
Alkaline Phosphatase小鼠单克隆抗体[HIS-1] to 6X His tag® -
宿主
Mouse -
偶联物
Alkaline Phosphatase -
经测试应用
适用于: WB, Dot blot, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant poly(Histidine)-tagged fusion protein.
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阳性对照
- Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
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常规说明
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.00
Preservative: 0.097% Sodium azide
Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
Primary antibody说明
Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors. -
克隆
单克隆 -
克隆编号
HIS-1 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49746于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/2000. Predicted molecular weight: 7 kDa.
|
Dot blot |
Use at an assay dependent dilution.
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ELISA |
Use at an assay dependent dilution.
|
说明 |
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WB
1/2000. Predicted molecular weight: 7 kDa. |
Dot blot
Use at an assay dependent dilution. |
ELISA
Use at an assay dependent dilution. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab49746 被引用在 6 文献中.
- Prasad KVSK et al. DNA-Binding Activity of CAMTA3 Is Essential for Its Function: Identification of Critical Amino Acids for Its Transcriptional Activity. Cells 12:N/A (2023). PubMed: 37566065
- Wu Z et al. Overexpression of fucosyltransferase 8 reverses the inhibitory effect of high-dose dexamethasone on osteogenic response of MC3T3-E1 preosteoblasts. PeerJ 9:e12380 (2021). PubMed: 34966572
- Yucel B et al. The copper-responsive ScsC protein of Salmonella promotes intramacrophage survival and interacts with the arginine sensor ArtI. FEBS J 287:3827-3840 (2020). PubMed: 32153092
- Bottermann M et al. Complement C4 Prevents Viral Infection through Capsid Inactivation. Cell Host Microbe 25:617-629.e7 (2019). PubMed: 30926239
- Henche AL et al. Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD. Extremophiles 18:905-13 (2014). PubMed: 25102813
- Hussack G et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . PubMed: 21216961