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AB184576

Alexa Fluor® 790 Anti-beta Actin抗体[mAbcam 8226] - Loading Control

Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control

5

(2 Reviews)

|

(2 Publications)

Mouse Monoclonal beta Actin antibody - conjugated to Alexa Fluor® 790. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications.

查看别名

Beta-actin, ACTB

1 Images
Western blot - Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB184576)
  • WB

Lab

Western blot - Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB184576)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184576 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

false

不同偶联物与剂型 (4)

  • Unconjugated

    Anti-beta Actin antibody [mAbcam 8226] - Loading Control

  • Carrier free

    Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free

  • HRP

    HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

  • 702 Alexa Fluor® 680

    Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

mAbcam 8226

亚型

IgG1

轻链类型

kappa

偶联物

Alexa Fluor® 790

激发波长/发射波长

Ex: 782nm, Em: 805nm

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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性能和储存信息

形式
Liquid
存储溶液
Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
See full target information ACTB
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 9:585-596 PubMed30949412

2019

Decreased miR-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5.

Applications

Unspecified application

Species

Unspecified reactive species

Guiling Yan,Yinhui Li,Lu Zhan,Shuhan Sun,Jihang Yuan,Tiantian Wang,Yupeng Yin,Zhihui Dai,Yiqing Zhu,Zhijing Jiang,Lin Liu,Yinxing Fan,Fu Yang,Wei Hu

International immunopharmacology 64:110-115 PubMed30172103

2018

Abietic acid attenuates IL-1β-induced inflammation in human osteoarthritis chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sining Kang,Jun Zhang,Ye Yuan
View all publications
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Abcam Product Promise

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详情请参阅我们的条款与条件。
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