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AB321399

Alexa Fluor® 750 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

Alexa Fluor® 750 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

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Rabbit Recombinant Monoclonal RAB5A antibody - conjugated to Alexa Fluor® 750. Early Endosome marker.

查看别名

RAB5, RAB5A, Ras-related protein Rab-5A

不同偶联物与剂型 (10)

  • Unconjugated

    Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • Carrier free

    Anti-Rab5 antibody [EPR21801] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 578 PE

    PE Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 660 APC

    APC Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • HRP

    HRP Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Rab5 antibody [EPR21801]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21801

亚型

IgG

偶联物

Alexa Fluor® 750

激发波长/发射波长

Ex: 749nm, Em: 775nm

不含载体蛋白

No

应用

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody could cross-react with RAB5C, but affinity is very low.

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.
Biological function summary

Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.

Pathways

The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.

Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
See full target information RAB5A
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