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AB321181

Alexa Fluor® 750 Anti-DNA Polymerase beta antibody [EPR12722(B)]

Alexa Fluor® 750 Anti-DNA Polymerase beta antibody [EPR12722(B)]

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Rabbit Recombinant Monoclonal DNA Polymerase beta antibody - conjugated to Alexa Fluor® 750.

查看别名

DNA polymerase beta, 5'-deoxyribose-phosphate lyase, AP lyase, 5'-dRP lyase, POLB

不同偶联物与剂型 (10)

  • Unconjugated

    Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • Carrier free

    Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free

  • 578 PE

    PE Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • 660 APC

    APC Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • HRP

    HRP Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-DNA Polymerase beta antibody [EPR12722(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-DNA Polymerase beta antibody [EPR12722(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12722(B)

亚型

IgG

偶联物

Alexa Fluor® 750

激发波长/发射波长

Ex: 749nm, Em: 775nm

不含载体蛋白

No

应用

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The human recommendation on the parent is based on the WB results. We do not guarantee IHC-P for human

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DNA polymerase beta often abbreviated as pol β serves as a repair enzyme that plays a role in the base excision repair (BER) pathway. This protein's mechanical function involves filling small gaps in the DNA created during repair processes by adding nucleotides. Its molecular weight is approximately 39 kDa. DNA polymerase beta is expressed extensively in the brain liver and testis indicating its importance in DNA repair in various cells. Other names for this protein include DNA pol beta and pol β. It synthesizes DNA using deoxyribonucleotide triphosphates as substrates and requires magnesium ions for activity.
Biological function summary

DNA polymerase beta helps to maintain genome integrity by repairing DNA lesions caused by oxidation alkylation or deamination. This protein often collaborates with other components of the DNA repair pathways but it does not directly form a larger protein complex focusing instead on its important function in BER. When DNA damage like single-strand breaks occur pol β carries out important gap-filling synthesis steps preparing the strand for subsequent ligation.

Pathways

DNA polymerase beta functions critically in base excision repair and acts alongside other important proteins like XRCC1. The BER pathway operates to correct DNA damage from endogenous sources preventing harmful mutations. This pathway is essential for maintaining cellular stability and protecting cells from apoptosis or malignant transformation that might occur if DNA damage propagates.

Mutations or dysfunctional activity within DNA polymerase beta have links to cancer and neurodegenerative diseases. For example errors in pol β's repair functions can lead to the persistence of DNA damage which can contribute to tumorigenesis. Furthermore its relationship with beta-actin has implications in cellular structural integrity and cancer metastasis. Dysfunctions within the BER pathway indicate potential contributions to neurodegenerative disorders where an accumulation of DNA damage is a common feature.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Repair polymerase that plays a key role in base-excision repair (PubMed : 10556592, PubMed : 9207062, PubMed : 9572863). During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap (PubMed : 10556592, PubMed : 17526740, PubMed : 9556598, PubMed : 9572863, PubMed : 9614142). Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase (PubMed : 9614142).
See full target information POLB
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