AB275946

Alexa Fluor® 700 Anti-CD28抗体[CD28.2]

Name: Alexa Fluor® 700 Anti-CD28抗体[CD28.2] (ab275946)| Abcam中文官网
Brand: Abcam Limited
SKU: AB275946
Price: 3038 CNY
Availability: InStock

Alexa Fluor® 700 Anti-CD28 antibody [CD28.2]

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Mouse Monoclonal CD28 antibody - conjugated to Alexa Fluor® 700. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD28 protein.

查看别名

CD28, T-cell-specific surface glycoprotein CD28, TP44

2 Images

Flow Cytometry - Alexa Fluor® 700 Anti-CD28 antibody [CD28.2] (AB275946)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 700 Anti-CD28 antibody [CD28.2] (AB275946)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab275946 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 700 Anti-CD28 antibody [CD28.2] (AB275946)

Separation of CD28 positive lymphocytes (red-filled) from CD28 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab275946 (4 μl reagent / 100 μl of peripheral whole blood).

不同偶联物与剂型 (8)

660 APC

APC Anti-CD28 antibody [CD28.2]
785 APC/Cy7®

APC/Cy7® Anti-CD28 antibody [CD28.2]
Biotin

Biotin Anti-CD28 antibody [CD28.2]
519 FITC

FITC Anti-CD28 antibody [CD28.2]
578 PE

PE Anti-CD28 antibody [CD28.2]
778 PE/Cy7®

PE/Cy7® Anti-CD28 antibody [CD28.2]
675 PerCP

PerCP Anti-CD28 antibody [CD28.2]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD28 antibody [CD28.2]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

CD28.2

亚型

IgG1

轻链类型

kappa

偶联物

Alexa Fluor® 700

激发波长/发射波长

Ex: 702nm, Em: 723nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.

P10747

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension</p>" } } }

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产品详情

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Size-exclusion chromatography

存储溶液

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.

Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 12196291, PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).

See full target information CD28

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Alexa Fluor® 700 Anti-CD28抗体[CD28.2]