重组Alexa Fluor® 647荧光Anti-Visfatin抗体[EPR21980] (ab318236)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)](https://www.abcam.cn/ps/products/318/ab318236/Images/ab318236-6-alexa-fluor-647-anti-visfatin-antibody-epr21980-immunocytochemistry-k-562.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR21980] to Visfatin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-Visfatin抗体[EPR21980]
参阅全部 Visfatin 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR21980] to Visfatin -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human breast cancer, Mouse kidney and Rat liver tissues. ICC/IF: HCT 116 and K-562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21980 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318236于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50.
|
|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
1/50. |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
组织特异性
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
序列相似性
Belongs to the NAPRTase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10135 Human
- Entrez Gene: 59027 Mouse
- Entrez Gene: 297508 Rat
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q99KQ4 Mouse
- SwissProt: Q80Z29 Rat
- Unigene: 489615 Human
see all -
别名
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in K-562 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)](https://www.abcam.cn/ps/products/318/ab318236/Images/ab318236-5-alexa-fluor-647-anti-visfatin-antibody-epr21980-immunocytochemistry-hct-116.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in HCT 116 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)](https://www.abcam.cn/ps/products/318/ab318236/Images/ab318236-3-alexa-fluor-647-anti-visfatin-antibody-epr21980-immunohistochemistry-rat-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution.
Positive staining on rat liver.
The section was incubated with ab318236 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)](https://www.abcam.cn/ps/products/318/ab318236/Images/ab318236-2-alexa-fluor-647-anti-visfatin-antibody-epr21980-immunohistochemistry-mouse-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution.
Positive staining on mouse kidney.
The section was incubated with ab318236 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)](https://www.abcam.cn/ps/products/318/ab318236/Images/ab318236-1-alexa-fluor-647-anti-visfatin-antibody-epr21980-immunohistochemistry-human-breast-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Visfatin with ab318236 at 1/50 (10.0 ug/ml) dilution.
Positive staining on human breast cancer.
The section was incubated with ab318236 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
-
![Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)](https://www.abcam.cn/ps/products/318/ab318236/Images/ab318236-4-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980] (ab318236)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab318236 尚未被引用在任何文献中。