Alexa Fluor® 647荧光Anti-VE Cadherin抗体[55-7H1] (ab272346)
![Flow Cytometry - Alexa Fluor® 647 Anti-VE Cadherin antibody [55-7H1] (ab272346)](https://www.abcam.cn/ps/products/272/ab272346/Images/ab272346-387974-Mouse-monoclonal-557H1-VE-Cadherin-Alexa-Fluor-647-flow-cytometry-huvec-human1.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-VE Cadherin antibody [55-7H1] (ab272346)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [55-7H1] to VE Cadherin
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Alexa Fluor® 647荧光Anti-VE Cadherin抗体[55-7H1]
参阅全部 VE Cadherin 一抗 -
描述
Alexa Fluor® 647荧光小鼠单克隆抗体[55-7H1] to VE Cadherin -
宿主
Mouse -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human VE Cadherin. Human endothelial cells.
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阳性对照
- Flow Cyt: HUVEC cells.
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常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Size exclusion -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
55-7H1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272346于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
组织特异性
Endothelial tissues and brain. -
序列相似性
Contains 5 cadherin domains. -
翻译后修饰
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
细胞定位
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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数据库链接
- Entrez Gene: 1003 Human
- Omim: 601120 Human
- SwissProt: P33151 Human
- Unigene: 76206 Human
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别名
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
图片
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Flow Cytometry - Alexa Fluor® 647 Anti-VE Cadherin antibody [55-7H1] (ab272346)
Flow cytometry surface staining pattern of HUVEC (Human umbilical vein endothelial cell line) cells stained using ab272346 at 4 μl reagent per milion cells in 100 μl of cell suspension. VE Cadherin also known as CD144.
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![Flow Cytometry - Alexa Fluor® 647 Anti-VE Cadherin antibody [55-7H1] (ab272346)](https://www.abcam.cn/ps/products/272/ab272346/Images/ab272346-387973-Mouse-monoclonal-557H1-VE-Cadherin-Alexa-Fluor-647-flow-cytometry-huvec-human2.jpg)
Flow Cytometry - Alexa Fluor® 647 Anti-VE Cadherin antibody [55-7H1] (ab272346)Separation of HUVEC (Human umbilical vein endothelial cell line) cells (red-filled) from HL-60 (Human promyelocytic leukemia cell line) cells (black-dashed) in flow cytometry analysis (surface staining) stained using ab272346 at 4 μl reagent per milion cells in 100 μl of cell suspension). VE Cadherin also known as CD144.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272346 尚未被引用在任何文献中。