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重组RabMAb

重组Alexa Fluor® 647荧光Anti-UBE2M/UBC12抗体[EPR5333] (ab225114)

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)
  • Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR5333] to UBE2M/UBC12
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Carrier Free PE Unconjugated

概述

  • 产品名称

    Alexa Fluor® 647荧光Anti-UBE2M/UBC12抗体[EPR5333]
    参阅全部 UBE2M/UBC12 一抗

  • 描述

    Alexa Fluor® 647荧光兔单克隆抗体[EPR5333] to UBE2M/UBC12

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: Ramos cells. Flow Cyt (intra): Ramos cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225114于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100.

This product gave a positive signal in Ramos cells fixed with 80% methanol (5 min)
Flow Cyt (Intra)
1/500.
说明
ICC/IF
1/100.

This product gave a positive signal in Ramos cells fixed with 80% methanol (5 min)
Flow Cyt (Intra)
1/500.

靶标

  • 功能

    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

  • 通路

    Protein modification; protein neddylation.

  • 序列相似性

    Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

  • 结构域

    Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt

  • 数据库链接

    • 别名

      • hUbc12 antibody
      • NEDD8 carrier protein antibody
      • NEDD8 conjugating enzyme Ubc12 antibody
      • NEDD8 protein ligase antibody
      • NEDD8-conjugating enzyme Ubc12 antibody
      • UBC-RS2 antibody
      • UBC12 antibody
      • UBC12_HUMAN antibody
      • ube2m antibody
      • ubiquitin carrier protein M antibody
      • Ubiquitin conjugating enzyme E2 M antibody
      • Ubiquitin-conjugating enzyme E2 M antibody
      • ubiquitin-protein ligase M antibody
      • yeast UBC12 homolog antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)

      Overlay histogram showing Ramos cells stained with ab225114 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225114, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)

      ab225114 staining UBE2M/UBC12 in Ramos cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225114 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)
      Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333] (ab225114)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab225114？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab225114 尚未被引用在任何文献中。

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