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AB323463

Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193]

Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193]

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Rabbit Recombinant Monoclonal TEAD4 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.

查看别名

RTEF1, TCF13L1, TEF3, TEAD4, Transcriptional enhancer factor TEF-3, TEA domain family member 4, Transcription factor 13-like 1, Transcription factor RTEF-1, TEAD-4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling TEAD4 with ab323463 at 1/50 (10.0 ug/ml) dilution.

Nuclear staining on human astrocytoma.
The section was incubated with ab323463 at 4°C overnight (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling TEAD4 with ab323463 at 1/50 (10.0 ug/ml) dilution.

Nuclear staining on human breast cancer.
The section was incubated with ab323463 at 4°C overnight (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TEAD4 with ab323463 at 1/50 (10.0 ug/ml) dilution.

Nuclear staining on human pancreas.
The section was incubated with ab323463 at 4°C overnight (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-TEAD4 antibody [EPR26046-193] (AB323463)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK-293T (Human embryonic kidney epithelial cell) cell pellet, (B) TEAD4 knockout HEK-293T cell pellet tissue labeling TEAD4 with ab323463 at 1/50 (10.0 ug/ml) dilution.

Positive staining on (A) Wild-type HEK293T cell pellet and no staining on (B) TEAD4 knockout HEK-293T cell pellet.
The section was incubated with ab323463 at 4°C overnight (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

不同偶联物与剂型 (2)

  • Unconjugated

    Anti-TEAD4/TEF-3 antibody [EPR26046-193]

  • Carrier free

    Anti-TEAD4/TEF-3 antibody [EPR26046-193] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR26046-193

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

反应种属

Human, Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Proteins & Peptides

AB238222

Recombinant Human TEAD4 protein (His tag)

SDS-PAGE - Recombinant Human TEAD4 protein (His tag) (AB238222)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TEAD4 also known as TEF-3 or TEF is a transcription factor that plays a significant mechanical role in gene regulation. It belongs to the TEAD family of proteins. TEAD4 has an approximate mass of 48 kDa and is ubiquitously expressed in various tissues. It interacts with different cofactors to regulate gene expression and influences several cellular processes. The protein's expression is especially noted in cancerous tissues emphasizing its role in cellular growth and proliferation.
Biological function summary

TEAD4 is important for embryonic development and cell fate determination. It forms a complex with YAP (Yes-associated protein) to regulate transcriptional activities. The TEAD4-YAP complex binds to DNA driving the expression of genes involved in cell proliferation and survival. TEAD4 also coordinates with _tef_1 and several other transcription factors to regulate genes necessary for development and differentiation influencing processes such as muscle and neural tissue formation.

Pathways

TEAD4 is primarily involved in the Hippo signaling pathway known to regulate organ size by controlling cell proliferation and apoptosis. TEAD4 interacts with proteins like YAP and TAZ which play integral roles within this pathway. The Hippo pathway influences cell growth and TEAD4 as a mediator of this action contributes to the balance between cell proliferation and apoptosis. TEAD4's role extends to pathways involving cell cycle regulation and response to cellular stress.

TEAD4 exhibits significant relevance in cancer and cardiovascular diseases. Overexpression or dysregulation of TEAD4 often through its interaction with YAP is linked with tumorigenesis in breast and liver cancers. It promotes cancer cell proliferation migration and invasion. In the cardiovascular system TEAD4 impacts heart development and function with implications in congenital heart defects. Its interaction with proteins like _tef_1 also highlights its role in these diseases making it a potential therapeutic target.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
See full target information TEAD4
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