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重组RabMAb

重组Alexa Fluor® 647荧光Anti-TCR delta抗体[EPR27043-16] (ab317716)

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)
  • Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)
  • Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)
  • Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR27043-16] to TCR delta
  • Suitable for: Flow Cyt, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free Unconjugated

概述

  • 产品名称

    Alexa Fluor® 647荧光Anti-TCR delta抗体[EPR27043-16]
    参阅全部 TCR delta 一抗

  • 描述

    Alexa Fluor® 647荧光兔单克隆抗体[EPR27043-16] to TCR delta

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • 经测试应用

    适用于: Flow Cyt, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Flow Cyt: Human PBMC cells. Flow Cyt (Intra): 293T (human embryonic kidney epithelial cell) transfected with a TRDC (human TCR delta) protein expression vector containing a myc-His tag.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317716于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
1/500.
Flow Cyt (Intra)
1/500.
说明
Flow Cyt
1/500.
Flow Cyt (Intra)
1/500.

靶标

  • 相关性

    Two distinct types of T cell antigen receptors have been identified: the alpha/beta heterodimer found on functional helper and cytotoxic T cells, and the gamma/delta heterodimer. The latter is first detected approximately 2 days before the appearance of cell surface alpha/beta heterodimer during T cell ontogeny. In adult thymus it is found mainly in the least mature cells. The gene shows systematic rearrangement in early thymocytes and appears to use V gene segments of the TCR alpha chain family, well before any C(alpha) message of protein is detected. RNA from this locus is expressed at a high level in early thymocytes and adult 'double-negative' cells, but not in mature T cell populations.

  • 数据库链接

    图片

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype / 293T (human embryonic kidney epithelial cell) transfected with a TRDC (human TCR delta) protein expression vector containing a myc-His tag(Up Right) / 293T transfected with a TRGC1 protein (human TCR gamma) expression vector(Down Left) / 293T transfected with an empty vector containing a myc-His tag(Down Right) cells labelling TCR delta with ab317716 at 1/500 dilution (0.1ug)/ Up Right, Down Left and Down Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    • Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)
      Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)

      Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab317716 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Gated on viable cells.

      Cells were co-stained with anti-CD3 conjugated to BV421.

    • Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)
      Flow Cytometry - Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)

      Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab317716 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Gated on viable cells.

      Cells were co-stained with anti-CD4 conjugated to BV421.

    • Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)
      Alexa Fluor® 647 Anti-TCR delta antibody [EPR27043-16] (ab317716)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317716？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317716 尚未被引用在任何文献中。

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