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AB325290

Alexa Fluor® 647 Anti-Syt1 antibody [7D5] - FluoTag®-X2

Alexa Fluor® 647 Anti-Syt1 antibody [7D5] - FluoTag®-X2

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Fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates featuring FluoTag® technology.

  • Quantitative and reliable readout in all fluorescence-based analyses
  • Defined number of fluorophores enhance brightness of signals.
  • Improved localization accuracy ideal for super resolution imaging.

查看别名

Synaptotagmin-1, Synaptotagmin I, p65, SytI, Syt1

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Syt1 antibody [7D5] - FluoTag®-X2 (AB325290)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Syt1 antibody [7D5] - FluoTag®-X2 (AB325290)

PFA-fixed hippocampal primary neuronal cultures from rats were stained with ab325290 at a 1/500 dilution. (A) Greyscale image of the staining performed with ab325290. (B) Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody. (C) DAPI signal of the depicted section. (D) Merge of A and C. False color representation of A in magenta, B in green and C in blue.

Other - Alexa Fluor® 647 Anti-Syt1 antibody [7D5] - FluoTag®-X2 (AB325290)
  • Other

Supplier Data

Other - Alexa Fluor® 647 Anti-Syt1 antibody [7D5] - FluoTag®-X2 (AB325290)

FluoTag®

FluoTag® reagents are alpaca-derived single-domain antibodies (sdAbs) directly conjugated to a defined number of fluorophores at known positions.

Depending on the specific target protein, they are available in up to three variants :
FluoTag®-Q : A single sdAb conjugated to a single fluorophore, ideal for quantitative readout.
FluoTag®-X2 : A single sdAb conjugated to two fluorophores, our standard variant.
FluoTag®-X4 : A blend of two different FluoTags®-X2 that bind to a single target. FluoTag®-X4 reagents effectively label the target (T) with 4 fluorophores in total, resulting in brighter signals.

关键信息

宿主种属

Alpaca

克隆

Monoclonal

克隆号

7D5

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

反应种属

Rat

应用

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

产品详情

FluoTags®

FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.

Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.

FluoTag®-X2 (One target - One sdAb – Two fluorophores (1:1:2)

FluoTag®-X2 reagents are composed of a single sdAb carrying two fluorophores per protein molecule. The maximal displacement of fluorophores from the antigen-binding surface is ~3 nm. FluoTag®-X2 reagents show good tissue penetration features.

Features:

  • High affinity monovalent binding + two fluorophores per sdAb for brighter signals
  • Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能和储存信息

形式
Liquid
纯化工艺
Size-exclusion chromatography
存储溶液
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1 month
推荐的短期储存条件
-20°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Calcium sensor that participates in triggering neurotransmitter release at the synapse (PubMed : 2333096, PubMed : 30107533). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.. (Microbial infection) Receptor for C.botulinum neurotoxin type B (BoNT/B, botB); interaction is improved in the presence of gangliosides (PubMed : 14504267, PubMed : 17167418, PubMed : 8144634). BoNT/B toxin binds to the membrane proximal vesicular domain of Syt1 (residues 32-51) (PubMed : 14504267, PubMed : 17167421).. (Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); unlike the case with BoNT/B, interaction is not improved in the presence of gangliosides (PubMed : 15123599, PubMed : 20219474). BoNT/G toxin binds to the vesicular domain of Syt1 (residues 32-53) (PubMed : 15123599, PubMed : 20219474).
See full target information Syt1
websiteProtocolBooklet
en

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