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AB198985

重组Alexa Fluor® 647荧光Anti-SUN2抗体[EPR6557]

Alexa Fluor® 647 Anti-SUN2 antibody [EPR6557]

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(1 Publication)

Rabbit Recombinant Monoclonal SUN2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

FRIGG, KIAA0668, RAB5IP, UNC84B, SUN2, SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Sad1/unc-84 protein-like 2, Rab5IP

3 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SUN2 antibody [EPR6557] (AB198985)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SUN2 antibody [EPR6557] (AB198985)

ab198985 staining SUN2 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198985 at 1/100 dilution (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml. This was followed by an incubation at room temperature for 1h with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, at 1μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SUN2 antibody [EPR6557] (AB198985)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SUN2 antibody [EPR6557] (AB198985)

Overlay histogram showing HeLa cells stained with ab198985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198985, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SUN2 antibody [EPR6557] (AB198985)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SUN2 antibody [EPR6557] (AB198985)

ab198985 staining SUN2 in SKNSH cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198985 at 1/100 dilution (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml. This was followed by an incubation at room temperature for 1h with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, at 1 μg/mL (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

不同偶联物与剂型 (7)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SUN2 antibody [EPR6557]

  • 578 PE

    PE Anti-SUN2 antibody [EPR6557]

  • 660 APC

    APC Anti-SUN2 antibody [EPR6557]

  • HRP

    HRP Anti-SUN2 antibody [EPR6557]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-SUN2 antibody [EPR6557]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-SUN2 antibody [EPR6557]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-SUN2 antibody [EPR6557]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6557

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor®; 647), is suitable for use as an isotype control with this antibody.</p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SUN2 also known as Sad1 and UNC84 domain containing 2 is a protein that plays a fundamental role in the positioning of the nucleus in cells. It has a mass of approximately 89 kDa. This protein localizes to the inner nuclear membrane where it is involved in tethering the nucleus to the cytoskeleton. SUN2 is mainly expressed in tissues exhibiting high mechanical stress such as muscle and bone tissue. Its structural role anchors the nuclear lamina to the cytoskeleton helping to maintain cellular integrity.
Biological function summary

SUN2 connects the nuclear interior with the cytoskeleton through the LINC complex (Linker of Nucleoskeleton and Cytoskeleton). This complex also includes other proteins like Nesprin and Lamins helping to transmit mechanical signals across the nuclear envelope. SUN2 plays an essential role in processes like cell division nuclear migration and chromosomal positioning. Its function in the LINC complex makes it a significant player in maintaining nuclear morphology and ensuring proper cell division.

Pathways

SUN2 is involved in the mechanotransduction and cell-cycle regulation pathways. In mechanotransduction SUN2 helps translate mechanical signals into biochemical ones therefore influencing gene expression. Related proteins like Nesprin-2 interact within the LINC complex and contribute to these pathways. In cell-cycle regulation the protein helps align the mitotic spindle through connection with components of the cytoskeleton supporting accurate cell division. These pathways demonstrate SUN2's contribution to cellular responses to mechanical stimuli and cell growth regulation.

Studies indicate that SUN2 mutations or abnormal expression levels are associated with muscular dystrophy and cancer. In muscular dystrophy defects in SUN2 can lead to impaired nuclear anchoring contributing to muscle weakness. It interacts with Lamin A/C and defects in these proteins are also linked to muscular dystrophies. In cancer SUN2 dysregulation may affect cellular architecture and chromosomal stability contributing to tumorigenesis. Understanding SUN2's roles and interactions offers potential pathways for therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2 : KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5.
See full target information SUN2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6416 PubMed37828059

2023

SUN1/2 controls macrophage polarization via modulating nuclear size and stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Jiao,Chuanchuan Li,Fenghua Guo,Jinjin Zhang,Hui Zhang,Zhifa Cao,Wenjia Wang,Wenbo Bu,Mobin Lin,Junhong Lü,Zhaocai Zhou
View all publications
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