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AB283504

重组Alexa Fluor® 647荧光Anti-SEC23B抗体[EPR22723-24]

Alexa Fluor® 647 Anti-SEC23B antibody [EPR22723-24]

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Rabbit Recombinant Monoclonal SEC23B antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Mouse samples.

查看别名

Protein transport protein Sec23B, SEC23-related protein B, Sec23b

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-SEC23B antibody [EPR22723-24] (AB283504)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-SEC23B antibody [EPR22723-24] (AB283504)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SEC23B with ab283504 at 1/100 (5.0 μg/ml) dilution. The section was incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab283504 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Positive staining on mouse cerebrum.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-SEC23B antibody [EPR22723-24] (AB283504)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-SEC23B antibody [EPR22723-24] (AB283504)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SEC23B with ab283504 at 1/50 (10.0 μ g/ml) dilution. The section was incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab283504 at 1/50 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Positive staining on mouse liver.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

不同偶联物与剂型 (3)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22723-24

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

反应种属

Mouse, Mouse

应用

Flow Cyt (Intra), IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

AB52484

Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

BOND RX™ Validated|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (AB52484)

  • 4
  • 14
Primary Antibodies

AB237964

Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade - BSA and Azide free (AB237964)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SEC23B also known as Protein Transport Protein Sec23B is a component of the coat protein complex II (COPII) which mediates transport from the endoplasmic reticulum to the Golgi apparatus. SEC23B weighs approximately 88 kDa and acts as a GTPase activating protein that regulates the COPII complex assembly. SEC23B broadly expresses in many tissues including high levels in the pancreas and liver suggesting its essential role in cellular transport processes.
Biological function summary

SEC23B functions in vesicular trafficking within cells. In the COPII complex it interacts with SEC24 proteins to form a structural framework critical for cargo selection and vesicle budding. SEC23B does not work alone and is part of a larger protein complex that includes SEC24 SAR1 and SEC13/31 which facilitates proper vesicle formation and trafficking. These interactions enable cells to accurately and efficiently transport necessary proteins and lipids through the secretory pathway.

Pathways

SEC23B is essential in the ER-to-Golgi transport pathway and plays a significant role in maintaining protein homeostasis. The secretory pathway requires SEC23B to cooperate with other proteins like SEC24C and SEC31A ensuring that cargo proteins are correctly packaged and delivered to their destination within the cell. This process is vital for the function of other pathways such as the unfolded protein response as it prevents the accumulation of misfolded proteins in the endoplasmic reticulum.

SEC23B mutations have links to Congenital Dyserythropoietic Anemia type II (CDA II) which manifests as ineffective erythropoiesis and improper glycosylation of red blood cell membrane proteins. SEC23B is also associated with pancreatic dysfunctions considering its high expression in the tissue although detailed mechanisms are less understood. In CDA II SEC23B's dysfunction affects its interaction with associated proteins like SEC24B disrupting normal vesicular trafficking and functional erythrocyte production.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.
See full target information Sec23b
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Abcam Product Promise

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