AB196175

重组Alexa Fluor® 647荧光Anti-S100 beta抗体[EP1576Y] - Astrocyte Marker

Name: Alexa Fluor® 647 Anti-S100 beta抗体[EP1576Y] - Astrocyte Marker (ab196175)| Abcam中文官网
Brand: Abcam Limited
SKU: AB196175
Price: 5009 CNY
Availability: InStock
Rating: 4 (1 reviews)

Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

RabMAb
Recombinant
了解详情

(1 Review)

(14 Publications)

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker - Alexa Fluor® 647 conjugated (ab196175) is a rabbit recombinant monoclonal antibody detecting S100 beta in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

查看别名

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)

ab196175 staining S100 beta in Malme-3M cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB196175)

ab196175 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

不同偶联物与剂型 (7)

HRP

HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
Unconjugated

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
Carrier free

Anti-S100 beta antibody [EP1576Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1576Y

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP1576Y is also available pre-conjugated to a variety of labels for your convenience – Anti-S100 beta Alexa Fluor® 647 [EP1576Y] - Astrocyte Marker (ab196175).

Other related products
We have a range of other formats of antibody clone [EP1576Y] also available for your convenience: ab52642, HRP - ab195535, Alexa Fluor® 647 - ab196175, Carrier free - ab215989, Alexa Fluor® 594 - ab307851, Alexa Fluor® 488 - ab310990, Alexa Fluor® 555 - ab313219, Alexa Fluor® 568 - ab313817

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.

Biological function summary

The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.

Pathways

S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.

S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed : 20950652, PubMed : 6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed : 20950652, PubMed : 6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed : 20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed : 22399290).

See full target information S100B

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8262 PubMed40998803

2025

Targeting prostaglandin E receptor 2 in Schwann cells inhibits inflammatory pain but not inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Nassini,Lorenzo Landini,Matilde Marini,Martina Chieca,Daniel Souza Monteiro de Araújo,Marco Montini,Pasquale Pensieri,Vittorio Donato Abruzzese,Gaetano De Siena,Jin Zhang,Elisa Bellantoni,Vincenzo De Giorgi,Antonia Romitelli,Giulia Brancolini,Raquel Tonello,Chloe J Peach,Alessandra Mastricci,Irene Scuffi,Martina Tesi,Dane D Jensen,Brian L Schmidt,Nigel W Bunnett,Francesco De Logu,Pierangelo Geppetti

Acta neuropathologica communications 13:45 PubMed40025578

2025

Development of CAR-T cell therapy for NF1/SWN-related nerve sheath tumor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Na Tang,Lei Cheng,Jiawei Hao,Beilei Xu,Xi Pan,Xiaofei Wei,Hao Wu,Haoyi Wang

Nature communications 15:10142 PubMed39587068

2024

Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa Titiz,Lorenzo Landini,Daniel Souza Monteiro de Araujo,Matilde Marini,Viola Seravalli,Martina Chieca,Pasquale Pensieri,Marco Montini,Gaetano De Siena,Benedetta Pasquini,Silvia Vannuccini,Luigi Francesco Iannone,Thiago M Cunha,Giulia Brancolini,Elisa Bellantoni,Irene Scuffi,Alessandra Mastricci,Martina Tesi,Mariarosaria Di Tommaso,Felice Petraglia,Pierangelo Geppetti,Romina Nassini,Francesco De Logu

International journal of molecular sciences 25: PubMed39125815

2024

SARS-CoV-2 Rapidly Infects Peripheral Sensory and Autonomic Neurons, Contributing to Central Nervous System Neuroinvasion before Viremia.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan D Joyce,Greyson A Moore,Poorna Goswami,Telvin L Harrell,Tina M Taylor,Seth A Hawks,Jillian C Green,Mo Jia,Matthew D Irwin,Emma Leslie,Nisha K Duggal,Christopher K Thompson,Andrea S Bertke

Current protocols 4:e1067 PubMed38857108

2024

Establishment of a Four-Cell In Vitro Blood-Brain Barrier Model With Human Primary Brain Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Johid R Malik,Ukamaka O Modebelu,Courtney V Fletcher,Anthony T Podany,Kimberly K Scarsi,Siddappa N Byrareddy,Robbyn K Anand,Shilpa Buch,Susmia Sil,Jennifer Le,John S Bradley,Ashley N Brown,Debapriya Sutar,Sean N Avedissian

Nature communications 14:3395 PubMed37296117

2023

Transparent tissue in solid state for solvent-free and antifade 3D imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Ting Hsiao,Hung-Jen Chien,Ya-Hsien Chou,Shih-Jung Peng,Mei-Hsin Chung,Tzu-Hui Huang,Li-Wen Lo,Chia-Ning Shen,Hsiu-Pi Chang,Chih-Yuan Lee,Chien-Chia Chen,Yung-Ming Jeng,Yu-Wen Tien,Shiue-Cheng Tang

Journal of neuroscience methods 392:109867 PubMed37116621

2023

A novel 4-cell in-vitro blood-brain barrier model and its characterization by confocal microscopy and TEER measurement.

Applications

Unspecified application

Species

Unspecified reactive species

Johid R Malik,Courtney V Fletcher,Anthony T Podany,Shetty Ravi Dyavar,Kimberly K Scarsi,Gwendolyn M Pais,Marc H Scheetz,Sean N Avedissian

Journal of biomedical science 30:28 PubMed37101198

2023

Acetaldehyde via CGRP receptor and TRPA1 in Schwann cells mediates ethanol-evoked periorbital mechanical allodynia in mice: relevance for migraine.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenzo Landini,Daniel Souza Monteiro de Araujo,Martina Chieca,Gaetano De Siena,Elisa Bellantoni,Pierangelo Geppetti,Romina Nassini,Francesco De Logu

JACC. Clinical electrophysiology 8:1191-1215 PubMed36182566

2022

The Heart's Pacemaker Mimics Brain Cytoarchitecture and Function: Novel Interstitial Cells Expose Complexity of the SAN.

Applications

Unspecified application

Species

Unspecified reactive species

Rostislav Bychkov,Magdalena Juhaszova,Miguel Calvo-Rubio Barrera,Lorenzo A H Donald,Christopher Coletta,Chad Shumaker,Kayla Moorman,Syevda Tagirova Sirenko,Alexander V Maltsev,Steven J Sollott,Edward G Lakatta

STAR protocols 3:101109 PubMed35106499

2022

A clearing-free protocol for imaging intact whole adipose tissue innervation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jake W Willows,Magdalena Blaszkiewicz,Kristy L Townsend

View all publications

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重组Alexa Fluor® 647荧光Anti-S100 beta抗体[EP1576Y] - Astrocyte Marker