重组Alexa Fluor® 647荧光Anti-ROR1抗体[EPR24451-141] (ab281331)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ROR1 antibody [EPR24451-141] (ab281331)](https://www.abcam.cn/ps/products/281/ab281331/Images/ab281331-2-anti-ror1-antibody-a647-flow-cytometry-panc-1-cells-human.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ROR1 antibody [EPR24451-141] (ab281331)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR24451-141] to ROR1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-ROR1抗体[EPR24451-141]
参阅全部 ROR1 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR24451-141] to ROR1 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): PANC-1 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
克隆
单克隆 -
克隆编号
EPR24451-141 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/2500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/2500. |
靶标
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功能
Tyrosine-protein kinase receptor whose role is not yet clear. -
组织特异性
Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
Contains 1 FZ (frizzled) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 kringle domain.
Contains 1 protein kinase domain. -
发展阶段
Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4919 Human
- Omim: 602336 Human
- SwissProt: Q01973 Human
- Unigene: 654491 Human
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别名
- dJ537F10.1 antibody
- Inactive tyrosine protein kinase transmembrane receptor ROR1 antibody
- MGC99659 antibody
see all
图片
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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ROR1 antibody [EPR24451-141] (ab281331)
Flow cytometry overlay histogram showing left PANC-1 positive cells and right negative MCF7 cells stained with ab281331 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab281331) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30 min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.
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![Alexa Fluor® 647 Anti-ROR1 antibody [EPR24451-141] (ab281331)](https://www.abcam.cn/ps/products/281/ab281331/Images/ab281331-456195-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 647 Anti-ROR1 antibody [EPR24451-141] (ab281331)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab281331 尚未被引用在任何文献中。