AB199093

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb
Recombinant
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(19 Publications)

Anti-Rabbit IgG, monoclonal [EPR25A] - Isotype Control- Alexa Fluor® 647 conjugated (ab199093) is a rabbit recombinant monoclonal antibody used in Flow Cytometry, ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

41 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199093 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/500 dilution (showing no signal) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt

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Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD11a with ab317290 at 1/5000 dilution (0.01ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : HeLa (PMID : 12036881). Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte, Left) / PANC-1 (human pancreatic epithelioid carcinoma epithelial cell, Right) cells labelling IL-13 receptor alpha 1 with ab315035 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control : Ramos. Gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt

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Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left) / U-937 (human histiocytic lymphoma monocyte, Right) cells labelling CLEC12A with ab317063 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells. Negative control : K-562.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt

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Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) treated with 500ng/ml ionomycin and 10ng/ml PMA for 3 hours (Lower left and right) / Untreated control (Upper left and right) cells labelling CD69 with ab325108 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control.

Cells were stained with anti-CD4 conjugated to Brilliant Violet 421.
Gated on viable lymphoid Cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Daudi (human Burkitt's lymphoma lymphoblast, Middle) / K-562 (human chronic myelogenous leukemia lymphoblast, Right) cells labelling MICB with ab325223 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.
Negative control : 293T, Daudi (PMID : 28154561).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Histone H3 with ab313347 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometry staining of Human PBMCs (top) or PBMCs treated with 1μg/ml LPS and 300ng/ml Brefeldin A for 18h (bottom), with ab316181 (right) or Alexa Fluor® 647 Rabbit IgG, monoclonal (ab199093) - Isotype Control (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316181 or Alexa Fluor® 647 Rabbit IgG, monoclonal (ab199093) - Isotype Control (1x 106 in 100 μl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD14.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling GC1q R with ab305041 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of Hela (human cervical adenocarcinoma epithelial cell, Left)/U-937 (human histiocytic lymphoma monocyte, Right) cells labelling CD18 with ab316944 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cell.
Negative control : HeLa

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / HEL (human erythroleukemia erythroblast, Right) cells labelling VEGF Receptor 3 with ab313899 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control : MCF7. Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling IL-10 with ab305044 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized hela(human cervical adenocarcinoma epithelial cell) cells labelling Ataxin 3 with ab315423 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) treated with 300nM thapsigargin for18hours (Red) / Untreated Huh7 (Dotted Red) cells labelling XBP1 with ab317358 at 1/5000 dilution(0.01ug)/Red and Dotted Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

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Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC12A with ab317063 at 1/5000 dilution (0.01ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control.

Gated on viable cells. Cells were co-stained with anti-CD3 conjugated to Alexa Fluor® 488.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG(human glioblastoma-astrocytoma epithelial cell) cells labelling Ataxin 3 with ab315423 at 1/50 dilution (1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling IL-12B with ab305042 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling ILT-4 with ab313898 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells are co-stained with CD14 conjugated to BV510. Gated on viable cells.

Flow Cyt

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Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Gated on viable cells. Cells were co-stained with anti-CD11b conjugated to BV421.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell) cells labelling JNK1 + JNK2 + JNK3 with ab315842 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 1%FBS for 3hours then 100nM Calyculin A for 10min (Red) / Untreated control (Dotted Red) cells labelling IKB alpha (phospho S36) with ab317485 at 1/5000 dilution (0.01ug) /Red and Dotted Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast, Left)/Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling Integrin alpha 4/CD49D with ab316939 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cell.
Negative control : K-562 (PMID : 16105875).

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD45 with ab315960 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : 293T. Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling Integrin alpha 4/CD49D with ab316939 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

human PBMC are co-stained with CD3 conjugated Alexa Fluor®488.
Gated on viable cell.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab316944 at 1/500 dilution (0.1ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Human PBMC are co-stained with CD4 conjugated BV421.
Gated on viable cell.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling TREM1 with ab316943 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Human PBMC are co-stained with CD3 conjugated Alexa Fluor®488.
Gated on viable cell.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MAPK14 KO HEK293T (human MAPK14 knock out human embryonic kidney epithelial cell, Left)/Parental HEK293T (Right) cells labelling p38 alpha/MAPK14 with ab316872 at 1/50 dilution (1 ug)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling T-bet / Tbx21 with ab315957 at 1/5000 dilution (0.01ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control.

Human PBMC are co-stained with CD19 conjugated to PE/Cy7, showing a distinct CD19 positive population with no cross reactivity to TBX21 positive cells.

Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD11a with ab317290 at 1/5000 dilution (0.01ug)/ Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control.

Gated on viable cells. Cells are co-stained with CD3 conjugated to Alexa Fluor® 488.

Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) treated with 1mM Pervanadate for 30min (Red) / Untreated Jurkat (Green) cells labelling CD3 zeta (phospho Y142) with ab310016 at 1/500 dilution (0.1 ug) /(Red and Green) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling KMT6 / EZH2 with ab317360 at 1/500 dilution(0.1ug)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Hsp70 with ab305046 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

不同偶联物与剂型 (13)

Unconjugated

Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Biotin

Biotin Isotype Control [EPR25A]
675 PerCP

PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
617 Alexa Fluor® 594

Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
578 PE

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control
603 Alexa Fluor® 568

Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
519 FITC

FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Carrier free

Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free
519 Alexa Fluor® 488

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
HRP

HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
421 Alexa Fluor® 405

Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
565 Alexa Fluor® 555

Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
660 APC

APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25A

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

应用

Flow Cyt (Intra), Flow Cyt, ICC/IF

applications

特异性

Please note Abcam have optimised the validation of this product. In our hands, we observe an increase in background signal intensity with the use of Triton X-100 and would recommend using an alternative permeabilisation method such as methanol or saponin.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "FlowCytIntra-species-checked": "", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Product Specifications
Alexa Fluor^® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt and ICC/IF .

Quality and Validation
Alexa Fluor^® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093) has been cited over 19 times in peer reviewed journals and is trusted by the scientific community.
Flourescent conjugated antibodies are ideal tools for multiplex IHC and flow cytometry experiments.
Alexa Fluor^® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093) has 19 independent reviews from customers.
Alexa Fluor^® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093) specifically detects rabbit IgG and is sold in 100 µL selling sizes.

Related Products
Conjugation-ready, carrier free format available for antibody clone (EPR25A) - ab210849.

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

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文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 3:100465 PubMed37323577

2023

Engineering CpG island DNA methylation in pluripotent cells through synthetic CpG-free ssDNA insertion.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Tompkins,Elizabeth Lizhar,Alireza Shokrani,Xiwei Wu,Jordan Berley,Diba Kamali,Deborah Hussey,Jonas Cerneckis,Tae Hyuk Kang,Jinhui Wang,Walter Tsark,Defu Zeng,Swetha Godatha,Rama Natarajan,Arthur Riggs

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300244 PubMed36938863

2023

Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Nikita Raj,Lilo Greune,Martin Kahms,Karina Mildner,Rico Franzkoch,Olympia Ekaterini Psathaki,Thomas Zobel,Dagmar Zeuschner,Jürgen Klingauf,Volker Gerke

Respiratory research 24:24 PubMed36691012

2023

RAGE inhibition alleviates lipopolysaccharides-induced lung injury via directly suppressing autophagic apoptosis of type II alveolar epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Xiong,Jiaying Dou,Jingyi Shi,Yuqian Ren,Chunxia Wang,Yucai Zhang,Yun Cui

Nature communications 13:6944 PubMed36376325

2022

A fluorogenic probe for predicting treatment response in non-small cell lung cancer with EGFR-activating mutations.

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Hui Deng,Qian Lei,Chengdi Wang,Zhoufeng Wang,Hai Chen,Gang Wang,Na Yang,Dan Huang,Quanwei Yu,Mengling Yao,Xue Xiao,Guonian Zhu,Cheng Cheng,Yangqian Li,Feng Li,Panwen Tian,Weimin Li

International journal of molecular sciences 23: PubMed35563257

2022

Oncolytic Herpes Simplex Virus Type 1 Induces Immunogenic Cell Death Resulting in Maturation of BDCA-1 Myeloid Dendritic Cells.

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Philipp Kalus,Jolien De Munck,Sarah Vanbellingen,Laura Carreer,Thessa Laeremans,Katrijn Broos,Inès Dufait,Julia K Schwarze,Ivan Van Riet,Bart Neyns,Karine Breckpot,Joeri L Aerts

Cancer communications (London, England) 41:1354-1372 PubMed34347390

2021

MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.

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Qingnan Wu,Weimin Zhang,Yan Wang,Qingjie Min,Hongyue Zhang,Dezuo Dong,Qimin Zhan

Pancreas 50:405-413 PubMed33835973

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Pancreatic Cancer Stem-Like Cells With High Calreticulin Expression Associated With Immune Surveillance.

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Yasuhiro Fujiwara,Ryouichi Tsunedomi,Kiyoshi Yoshimura,Satoshi Matsukuma,Nobuyuki Fujiwara,Mitsuo Nishiyama,Shinsuke Kanekiyo,Hiroto Matsui,Yoshitaro Shindo,Yukio Tokumitsu,Shin Yoshida,Michihisa Iida,Nobuaki Suzuki,Shigeru Takeda,Tatsuya Ioka,Shoichi Hazama,Hiroaki Nagano

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2021

Targeted replacement of full-length CFTR in human airway stem cells by CRISPR-Cas9 for pan-mutation correction in the endogenous locus.

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Sriram Vaidyanathan,Ron Baik,Lu Chen,Dawn T Bravo,Carlos J Suarez,Shayda M Abazari,Ameen A Salahudeen,Amanda M Dudek,Christopher A Teran,Timothy H Davis,Ciaran M Lee,Gang Bao,Scott H Randell,Steven E Artandi,Jeffrey J Wine,Calvin J Kuo,Tushar J Desai,Jayakar V Nayak,Zachary M Sellers,Matthew H Porteus

Nature protocols 16:1629-1646 PubMed33495627

2021

Nuclei isolation of multiple brain cell types for omics interrogation.

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Alexi Nott,Johannes C M Schlachetzki,Bethany R Fixsen,Christopher K Glass

Cancer cell 37:371-386.e12 PubMed32109374

2020

Proteome Instability Is a Therapeutic Vulnerability in Mismatch Repair-Deficient Cancer.

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Daniel J McGrail,Jeannine Garnett,Jun Yin,Hui Dai,David J H Shih,Truong Nguyen Anh Lam,Yang Li,Chaoyang Sun,Yongsheng Li,Rosemarie Schmandt,Ji Yuan Wu,Limei Hu,Yulong Liang,Guang Peng,Eric Jonasch,David Menter,Melinda S Yates,Scott Kopetz,Karen H Lu,Russell Broaddus,Gordon B Mills,Nidhi Sahni,Shiaw-Yih Lin

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