重组Alexa Fluor® 647荧光Anti-QKI抗体[EPR7306] (ab223419)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-QKI antibody [EPR7306] (ab223419)](https://www.abcam.cn/ps/products/223/ab223419/Images/ab223419-287034-ICCAP00367421.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-QKI antibody [EPR7306] (ab223419)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR7306] to QKI
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-QKI抗体[EPR7306]
参阅全部 QKI 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR7306] to QKI -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: Neuro-2a cells
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7306 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223419于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in Neuro-2a cells fixed with 4% formaldehyde (10 min) |
| 说明 |
|---|
|
ICC/IF
1/100. This product gave a positive signal in Neuro-2a cells fixed with 4% formaldehyde (10 min) |
靶标
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功能
RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. -
组织特异性
Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients. -
序列相似性
Contains 1 KH domain. -
结构域
The KH domain and the Qua2 region are involved in RNA binding. -
翻译后修饰
Methylated by PRMT1.
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 9444 Human
- Entrez Gene: 19317 Mouse
- Entrez Gene: 499022 Rat
- Omim: 609590 Human
- SwissProt: Q96PU8 Human
- SwissProt: Q9QYS9 Mouse
- SwissProt: Q91XU1 Rat
- Unigene: 510324 Human
see all -
别名
- DKFZp586I0923 antibody
- HKQ antibody
- Homolog of mouse quaking QKI KH domain RNA binding protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-QKI antibody [EPR7306] (ab223419)
ab223419 staining QK1 in Neuro-2a cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223419 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-QKI antibody [EPR7306] (ab223419)](https://www.abcam.cn/ps/products/223/ab223419/Images/ab223419-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-QKI antibody [EPR7306] (ab223419)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223419 尚未被引用在任何文献中。