AB325114

Alexa Fluor® 647 Anti-PMCA2 antibody [EPR28923-56]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Calcium Pump PMCA2 ATPase antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.

Pmca2, Plasma membrane calcium-transporting ATPase 2, PMCA2, Plasma membrane calcium ATPase isoform 2, Plasma membrane calcium pump isoform 2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PMCA2 antibody [EPR28923-56] (AB325114)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PMCA2 antibody [EPR28923-56] (AB325114)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PMCA2 with ab325114 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on mouse lung.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PMCA2 antibody [EPR28923-56] (AB325114)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PMCA2 with ab325114 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on rat lung.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PMCA2 antibody [EPR28923-56] (AB325114)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PMCA2 with ab325114 at 1/50 (10.0 ug/ml) dilution.

Positive staining on mouse cerebrum.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-PMCA2 antibody [EPR28923-56] (AB325114)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PMCA2 with ab325114 at 1/50 (10.0 ug/ml) dilution.

Positive staining on rat cerebrum.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

不同偶联物与剂型 (5)

Unconjugated

Anti-PMCA2 antibody [EPR28923-56]
Carrier free

Anti-PMCA2 antibody [EPR28923-56] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Calcium Pump PMCA2 ATPase antibody [EPR28923-56]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Calcium Pump PMCA2 ATPase antibody [EPR28923-56]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Calcium Pump PMCA2 ATPase antibody [EPR28923-56]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR28923-56

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

反应种属

Mouse, Rat, Human, Mouse, Rat

应用

ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Not suitable for human IHC-P and rat ICC. This antibody weakly cross-react with PMCA1, PMCA3 and PMCA4 overexpression lysates.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Calcium Pump PMCA2 ATPase also known as PMCA2 or Ca-ATPase pump plays an important role in the active transport of calcium ions from the cytoplasm across the plasma membrane. It functions by hydrolyzing ATP to provide the energy needed for this process. PMCA2 belongs to the family of P-type ATPases and has a molecular mass of approximately 135 kDa. This pump is prominently expressed in the brain and inner ear particularly in the cerebellum and cochlea highlighting its importance in neuronal and auditory functions.

Biological function summary

PMCA2 regulates intracellular calcium concentration which is critical for cellular signaling and homeostasis. It does not form part of a larger complex but works independently to balance calcium levels. In neuronal cells efficient calcium clearance is necessary for synaptic activity and PMCA2 helps in maintaining the rapid calcium ion flux during neuronal firing. The presence of PMCA2 in auditory hair cells signifies its role in cochlear function and hearing sensitivity by aiding the processing of sound signals through calcium modulation.

Pathways

PMCA2 integrates into calcium signaling pathways and interacts with calmodulin which enhances its activity. Its function in the calmodulin-calcium signaling pathway is essential for processes such as neurotransmitter release and muscle contraction. In association with proteins like calmodulin PMCA2 ensures precise calcium dynamics safeguarding against cytotoxicity triggered by excessive calcium accumulation. The coordination with calmodulin formulates a pivotal connection within various signaling cascades.

PMCA2 mutations or dysfunctions have associations with neurological disorders and hearing impairments. Research links PMCA2 dysregulation to conditions such as spinocerebellar ataxia and hereditary hearing loss. In these scenarios PMCA2 interacts with molecular components that when impaired disrupt the normal calcium homeostasis. Such interruptions can lead to neurodegeneration or compromised auditory function underlining the significance of PMCA2 in maintaining health and preventing disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels in specialized cells of cerebellar circuit and vestibular and cochlear systems (PubMed : 17234811, PubMed : 9668038). Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment. Has fast activation and Ca(2+) clearance rate suited to control fast neuronal Ca(2+) dynamics (PubMed : 17409239, PubMed : 20083513). At parallel fiber to Purkinje neuron synapse, mediates presynaptic Ca(2+) efflux in response to climbing fiber-induced Ca(2+) rise. Provides for fast return of Ca(2+) concentrations back to their resting levels, ultimately contributing to long-term depression induction and motor learning (PubMed : 17409239, PubMed : 20083513). Plays an essential role in hearing and balance. In cochlear hair cells, shuttles Ca(2+) ions from stereocilia to the endolymph and dissipates Ca(2+) transients generated by the opening of the mechanoelectrical transduction channels. Regulates Ca(2+) levels in the vestibular system, where it contributes to the formation of otoconia (By similarity) (PubMed : 9668038). Regulates Ca(2+) signaling through dissipation of Ca(2+) transients generated by store-operated channels (By similarity). In lactating mammary gland, allows for the high content of Ca(2+) ions in the milk (PubMed : 15302868).

See full target information Atp2b2

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Alexa Fluor® 647 Anti-PMCA2 antibody [EPR28923-56]