AB199836

重组Alexa Fluor® 647荧光Anti-PBR抗体[EPR5384]

Alexa Fluor® 647 Anti-PBR antibody [EPR5384]

RabMAb
Recombinant
KO Validated
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal PBR antibody - conjugated to Alexa Fluor® 647. Suitable for ICC and reacts with Human samples. Cited in 3 publications.

查看别名

BZRP, MBR, TSPO, Translocator protein, Mitochondrial benzodiazepine receptor, PKBS, Peripheral-type benzodiazepine receptor, PBR

2 Images

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (AB199836)

ab199836 staining PBR in wild-type HAP1 cells (top panel) and PBR knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab199836 at 1/500 dilution (shown in red) and ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (AB199836)

ab199836 staining PBR in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

不同偶联物与剂型 (5)

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-PBR antibody [EPR5384]
578 PE

PE Anti-PBR antibody [EPR5384]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PBR antibody [EPR5384]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PBR antibody [EPR5384]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-PBR antibody [EPR5384]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5384

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

反应种属

Human

应用

ICC

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).</p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.012% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PBR also known as the Peripheral Benzodiazepine Receptor is a protein predominantly found in the outer mitochondrial membrane. It has alternate names like Translocator Protein (TSPO) and the mass of this protein is approximately 18 kDa. PBR is expressed in various tissues but shows high levels in steroidogenic tissues like adrenal glands as well as in the brain heart liver and kidneys. The abundant presence in these tissues highlights its importance in a variety of physiological functions.

Biological function summary

PBR interacts with cholesterol for the synthesis of steroid hormones making it important for steroidogenesis. PBR is a part of the Mitochondrial Permeability Transition Pore complex (MPTP) involved in regulating the transport of molecules across the mitochondrial membrane. Through its association with the MPTP PBR plays a significant role in mitochondrial functions such as apoptosis and energy metabolism. The interaction with other molecules also includes the binding with benzodiazepines impacting processes like immune response and cell proliferation.

Pathways

PBR plays a role in the steroid biosynthesis and apoptosis pathways. It interfaces with the StAR (Steroidogenic Acute Regulatory) protein to facilitate cholesterol transport into mitochondria the initial step in steroid hormone production. PBR is also involved in pathways that regulate apoptosis and mitochondrial function linking it to different cellular processes through interactions with proteins like VDAC (Voltage-Dependent Anion Channel).

PBR has relevance to conditions such as neurodegenerative diseases and cancer. PBR expression changes in disorders like Alzheimer's disease where it might reflect mitochondrial dysfunctions. It has also been associated with certain cancers where aberrant PBR activity might contribute to altered cell proliferation and apoptosis. Proteins like caspases are involved in the apoptotic pathways connected with PBR highlighting its involvement in disease processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed : 24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed : 1847678).

See full target information TSPO

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadi8986 PubMed37889970

2023

Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Laura M Bartos,Sabrina V Kirchleitner,Zeynep Ilgin Kolabas,Stefanie Quach,Alexander Beck,Julia Lorenz,Jens Blobner,Stephan A Mueller,Selin Ulukaya,Luciano Hoeher,Izabela Horvath,Karin Wind-Mark,Adrien Holzgreve,Viktoria C Ruf,Lukas Gold,Lea H Kunze,Sebastian T Kunte,Philipp Beumers,Ha Eun Park,Melissa Antons,Artem Zatcepin,Nils Briel,Leonie Hoermann,Rebecca Schaefer,Denise Messerer,Peter Bartenstein,Markus J Riemenschneider,Simon Lindner,Sibylle Ziegler,Jochen Herms,Stefan F Lichtenthaler,Ali Ertürk,Joerg C Tonn,Louisa von Baumgarten,Nathalie L Albert,Matthias Brendel

European journal of nuclear medicine and molecular 47:2131-2141 PubMed31960097

2020

Initial experience with [F]DPA-714 TSPO-PET to image inflammation in primary angiitis of the central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Backhaus,Wolfgang Roll,Carolin Beuker,Bastian Zinnhardt,Robert Seifert,Christian Wenning,Michel Eisenblätter,Christian Thomas,Antje Schmidt-Pogoda,Daniel Strunk,Stefan Wagner,Andreas Faust,Frank Tüttelmann,Albrecht Röpke,Andreas H Jacobs,Walter Stummer,Heinz Wiendl,Sven G Meuth,Michael Schäfers,Oliver Grauer,Jens Minnerup

Theranostics 9:1523-1537 PubMed31037121

2019

Molecular Imaging of Immune Cell Dynamics During De- and Remyelination in the Cuprizone Model of Multiple Sclerosis by [F]DPA-714 PET and MRI.

Applications

Unspecified application

Species

Unspecified reactive species

Bastian Zinnhardt,Michaël Belloy,Inga B Fricke,Jasmien Orije,Caroline Guglielmetti,Sven Hermann,Stefan Wagner,Michael Schäfers,Annemie Van der Linden,Andreas H Jacobs

View all publications

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