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AB225100

重组Alexa Fluor® 647荧光Anti-Olig2抗体[EPR2673]

Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

5

(1 Review)

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(9 Publications)

Anti-Olig2 antibody [EPR2673] - Alexa Fluor® 647 conjugated (ab225100) is a rabbit recombinant monoclonal antibody detecting Olig2 in IHC-Fr. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

查看别名

BHLHB1, BHLHE19, PRKCBP2, RACK17, OLIG2, Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)

Immunofluorescence staining of Olig2 in a section of frozen rat cerebellum.

The section was fixed using 10% formaldehyde in 1X PBS for 10 minutes. No antigen retrieval step was performed prior to staining. Performed on a Leica BONDTM. The section was incubated at room temperature for 1 hour with ab225100 at 1/100 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB225100)

Immunohistochemistry (Frozen sections) analysis of Mouse cerebrum (fresh frozen) labeling Olig2 with ab225100 at 1/50. The section was fixed using 4% PFA and permeabilised with 0.2% Triton X-100. Nuclear counterstain was DAPI. Positive staining on mouse cerebrum.

不同偶联物与剂型 (6)

  • Unconjugated

    Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

  • Carrier free

    Anti-Olig2 antibody [EPR2673] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Olig2 antibody [EPR2673]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2673

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

反应种属

Rat, Human, Mouse

应用

IHC-Fr, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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替代产品

Primary Antibodies

AB136253

Anti-Olig2 antibody - Oligodendrocyte Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody - Oligodendrocyte Marker (AB136253)

  • 4
  • 1
Primary Antibodies

AB183487

Anti-Olig2 (phospho S10 + S13 + S14) antibody

Western blot - Anti-Olig2 (phospho S10 + S13 + S14) antibody (AB183487)

  • 5
  • 2
Primary Antibodies

AB237816

Anti-Olig2 antibody [OLIG2/2400] - BSA and Azide free

Protein Array - Anti-Olig2 antibody [OLIG2/2400] - BSA and Azide free (AB237816)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB190565

Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190565)

  • 5
  • 7
Primary Antibodies

AB196149

Alexa Fluor® 647 Anti-ERG antibody [EPR3864]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (AB196149)

  • 4
  • 3
Primary Antibodies

AB7349

Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker

BOND RX™ Validated|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (AB7349)

  • 4
  • 23
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50 - 1/100", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

What is this antibody validated in?
Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] (ab225100) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

Related products
Antibody clone EPR2673 is also available pre-conjugated to a variety of labels for your convenience – Anti-Olig2 Alexa Fluor® 647 [EPR2673] (ab225100).

Other related products
We have a range of other formats of antibody clone [EPR2673] also available for your convenience: ab109186, Carrier free - ab220796, Alexa Fluor® 488 - ab225099, Alexa Fluor® 647 - ab225100, Alexa Fluor® 594 - ab300729

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Olig2 also known as Oligo2 or OLIG2 is a protein with a molecular mass of around 32 kDa. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which play roles in neurodevelopment. Olig2 is expressed mainly in the central nervous system particularly in oligodendrocyte progenitor cells and motor neuron progenitors. As an important marker Olig2 staining is often used in studies involving these cell types to understand differentiation processes.
Biological function summary

Olig2 regulates gene expression important for the proper development of the central nervous system. It functions both independently and as a part of transcriptional complexes to influence the fate of neural progenitors. In particular its presence decides whether these progenitors will differentiate into neurons astrocytes or oligodendrocytes. Among the proteins it interacts with are factors that modulate neurogenesis and myelination highlighting its importance in maintaining normal brain homeostasis.

Pathways

Olig2 plays an important role in the oligodendrocyte differentiation and motor neuron generation pathways. It works closely with proteins such as SOX10 and NKX2.2 to regulate oligodendrocyte maturation. In the motor neuron pathway Olig2 interacts with proteins like NKX6.1 and LHX3 coordinating the development of motor neurons. These pathways demonstrate Olig2's function as a critical transcription factor influencing neural development and plasticity.

Abnormalities in Olig2 expression or function relate to conditions such as gliomas and Multiple Sclerosis (MS). Elevated levels of Olig2 are often seen in gliomas where it might cooperate with proteins like IDH1 to drive tumor growth. In MS the impairment of oligodendrocyte function can link back to disruptions in normal Olig2 activity or expression. These associations make Olig2 a significant target for potential therapeutic interventions in neurological conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
See full target information OLIG2
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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:676-686 PubMed40770097

2025

Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1.

Applications

Unspecified application

Species

Unspecified reactive species

Diankun Yu,Samhita Jain,Andi Wangzhou,Beika Zhu,Wenyuan Shao,Elena J Coley-O'Rourke,Stacy De Florencio,JaeYeon Kim,Jennifer Ja-Yoon Choi,Mercedes F Paredes,Tomasz J Nowakowski,Eric J Huang,Xianhua Piao

Journal of proteome research 23:3847-3857 PubMed39056441

2024

FANS Unfixed: Isolation and Proteomic Analysis of Mouse Cell Type-Specific Brain Nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Bedwell,Myrto Mavrotas,Nikita Demchenko,Reuben M Yaa,Brittannie Willis,Zuzana Demianova,Nelofer Syed,Harry J Whitwell,Alexi Nott

Nature neuroscience 26:1701-1712 PubMed37749256

2023

IL-12 sensing in neurons induces neuroprotective CNS tissue adaptation and attenuates neuroinflammation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Myrto Andreadou,Florian Ingelfinger,Donatella De Feo,Teresa L M Cramer,Selma Tuzlak,Ekaterina Friebel,Bettina Schreiner,Pascale Eede,Shirin Schneeberger,Maria Geesdorf,Frederike Ridder,Christina A Welsh,Laura Power,Daniel Kirschenbaum,Shiva K Tyagarajan,Melanie Greter,Frank L Heppner,Sarah Mundt,Burkhard Becher

Immunity 56:2152-2171.e13 PubMed37582369

2023

Human microglia maturation is underpinned by specific gene regulatory networks.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Z Han,Rick Z Li,Emily Hansen,Samantha Trescott,Bethany R Fixsen,Celina T Nguyen,Cristina M Mora,Nathanael J Spann,Hunter R Bennett,Olivier Poirion,Justin Buchanan,Anna S Warden,Bing Xia,Johannes C M Schlachetzki,Martina P Pasillas,Sebastian Preissl,Allen Wang,Carolyn O'Connor,Shreya Shriram,Roy Kim,Danielle Schafer,Gabriela Ramirez,Jean Challacombe,Samuel A Anavim,Avalon Johnson,Mihir Gupta,Ian A Glass,Michael L Levy,Sharona Ben Haim,David D Gonda,Louise Laurent,Jennifer F Hughes,David C Page,Mathew Blurton-Jones,Christopher K Glass,Nicole G Coufal

Molecular cell 82:4647-4663.e8 PubMed36525955

2022

HIV integration in the human brain is linked to microglial activation and 3D genome remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Amara L Plaza-Jennings,Aditi Valada,Callan O'Shea,Marina Iskhakova,Benxia Hu,Behnam Javidfar,Gabriella Ben Hutta,Tova Y Lambert,Jacinta Murray,Bibi Kassim,Sandhya Chandrasekaran,Benjamin K Chen,Susan Morgello,Hyejung Won,Schahram Akbarian

Cell 185:2184-2199.e16 PubMed35649412

2022

Glioma progression is shaped by genetic evolution and microenvironment interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Frederick S Varn,Kevin C Johnson,Jan Martinek,Jason T Huse,MacLean P Nasrallah,Pieter Wesseling,Lee A D Cooper,Tathiane M Malta,Taylor E Wade,Thais S Sabedot,Daniel Brat,Peter V Gould,Adelheid Wöehrer,Kenneth Aldape,Azzam Ismail,Santhosh K Sivajothi,Floris P Barthel,Hoon Kim,Emre Kocakavuk,Nazia Ahmed,Kieron White,Indrani Datta,Hyo-Eun Moon,Steven Pollock,Christine Goldfarb,Ga-Hyun Lee,Luciano Garofano,Kevin J Anderson,Djamel Nehar-Belaid,Jill S Barnholtz-Sloan,Spyridon Bakas,Annette T Byrne,Fulvio D'Angelo,Hui K Gan,Mustafa Khasraw,Simona Migliozzi,D Ryan Ormond,Sun Ha Paek,Erwin G Van Meir,Annemiek M E Walenkamp,Colin Watts,Tobias Weiss,Michael Weller,Karolina Palucka,Lucy F Stead,Laila M Poisson,Houtan Noushmehr,Antonio Iavarone,Roel G W Verhaak

Nature neuroscience 25:285-294 PubMed35210624

2022

Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Ratz,Leonie von Berlin,Ludvig Larsson,Marcel Martin,Jakub Orzechowski Westholm,Gioele La Manno,Joakim Lundeberg,Jonas Frisén

Nature protocols 16:1629-1646 PubMed33495627

2021

Nuclei isolation of multiple brain cell types for omics interrogation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexi Nott,Johannes C M Schlachetzki,Bethany R Fixsen,Christopher K Glass

Frontiers in molecular neuroscience 13:232 PubMed33343295

2020

Gene Therapy Vector Encoding Neuropeptide Y and Its Receptor Y2 for Future Treatment of Epilepsy: Preclinical Data in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Alicja Szczygieł,Kira Iben Danielsen,Esbjörn Melin,Søren Hofman Rosenkranz,Stanislava Pankratova,Annika Ericsson,Karin Agerman,Merab Kokaia,David Paul Drucker Woldbye
View all publications
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