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重组RabMAb

重组Alexa Fluor® 647荧光Anti-NDUFB8抗体[EPR15961] (ab317711)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
  • Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR15961] to NDUFB8
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free Unconjugated

概述

  • 产品名称

    Alexa Fluor® 647荧光Anti-NDUFB8抗体[EPR15961]
    参阅全部 NDUFB8 一抗

  • 描述

    Alexa Fluor® 647荧光兔单克隆抗体[EPR15961] to NDUFB8

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human kidney, Mouse brain and Rat kidney tissues. ICC/IF: C6, HeLa and NIH/3T3 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317711于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/50.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
ICC/IF
1/50.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • 序列相似性

    Belongs to the complex I NDUFB8 subunit family.

  • 细胞定位

    Mitochondrion inner membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ASHI antibody
      • CI-ASHI antibody
      • Complex I ASHI subunit antibody
      • Complex I-ASHI antibody
      • mitochondrial antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
      • NADH-ubiquinone oxidoreductase ASHI subunit antibody
      • NDUB8_HUMAN antibody
      • NDUFB8 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB8 with ab317711 at 1/100 (5.0 ug/ml) dilution.

      Positive staining on human kidney.The section was incubated with ab317711 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Counterstained with DAPI.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

      Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NDUFB8 with ab317711 at 1/100 (5.0 ug/ml) dilution.

      Positive staining on mouse brain.The section was incubated with ab317711 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Counterstained with DAPI.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NDUFB8 with ab317711 at 1/100 (5.0 ug/ml) dilution.

      Positive staining on rat kidney.The section was incubated with ab317711 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Counterstained with DAPI.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling NDUFB8 with ab317711 at 1/50 (10.0 ug/ml) dilution (Magenta).

      Confocal image showing cytoplasmic staining in C6 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

      ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling NDUFB8 with ab317711 at 1/50 (10.0 ug/ml) dilution (Magenta).

      Confocal image showing cytoplasmic staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

      ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling NDUFB8 with ab317711 at 1/50 (10.0 ug/ml) dilution (Magenta).

      Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

      ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

    • Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)
      Alexa Fluor® 647 Anti-NDUFB8 antibody [EPR15961] (ab317711)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317711？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317711 尚未被引用在任何文献中。

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