Alexa Fluor® 647荧光Anti-Myc tag抗体[9E10]

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Nucleoprotein expression vector containing a myc-tag. cells labelling HCoV-OC43 Nucleoprotein with ab309964 at 1/100 (10.87 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with HCoV-OC43 Nucleoprotein expression vector containing a myc tag. No staining on HEK-293T transfected with empty vector containing a myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RIP3 (phospho T231) with ab324983 at 1/200 (2.6 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a mouse RIP3 expression vector containing a myc-His-tag® and no staining in 293T cells transfected with a mouse RIPK3(T231A, S232A) expression vector containing a myc-His-tag® (shown in green) . The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 (5 ug/ml) dilution (Magenta).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a CBX1-V5-tagged expression vector containing a His-tag. cells labelling V5 tag with ab314555 at 1/500 (1.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1015 (2ug/ml) dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with V5 tag protein expression vector containing a myc tag, no staining on HEK-293T transfected with empty vector containing a His-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1015 (2ug/ml) dilution.

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab223895 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Alexa Fluor^® 647 (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor^® 647 conjugated Myc-tag antibody (ab223895) and Alexa Fluor^® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling hMPV prefusion F with ab320652 at 1/1000 (0.517 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a hMVP prefusion F expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a hRSV prefusion F expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

ab223895 staining Myc-tag (pseudocolored in white) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223895 at 1/100 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor^® 488 conjugated control Myc-tag antibody (different clone) is shown in green.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• Flow Cyt

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Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Flow cytometric analysis 4% Paraformaldehyde fixed 90% methanol permeabilised HEK-293T (Human embryonic kidney epithelial cell) cells transfected with a SARS-CoV-2 nsp1 (Left) or SARS-CoV2 nsp13 (Right) expression vector containing a myc tag labelling SARS-CoV-2 nsp13 with ab286909 at 1/50 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/5000 dilution was used as the secondary antibody. Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895) was used to label transfected cells.

• Flow Cyt

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Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Flow Cytometry analysis of HEK-293T (Human embryonic kidney epithelial cell) cells transfected with a SARS-CoV-2 nsp9 (Left) or SARS-CoV2 nsp15 (Right) expression vector containing a myc tag labeling SARS-CoV2 nsp15 with ab284629 at 1/500 dilution (left and right). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/5000 dilution. Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895) was used to label transfected cells.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labelling Stra8 with ab308124 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with a mouse STRA8 expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (10 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (AB223895)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Nucleoprotein expression vector containing a myc-tag. cells labelling HCoV-OC43 Nucleoprotein with ab309966 at 1/90 (10.36 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 dilution (Green). Confocal image showing cytoplasmic staining in 239T cells transfected with HCoV-OC43 Nucleoprotein expression vector containing a myc tag. No staining on HEK-293T transfected with empty vector containing a myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 dilution.