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AB325302

Alexa Fluor® 647 Anti-mEOS antibody [1E8] - FluoTag®-X2

Alexa Fluor® 647 Anti-mEOS antibody [1E8] - FluoTag®-X2

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Fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates featuring FluoTag® technology.

  • Quantitative and reliable readout in all fluorescence-based analyses
  • Defined number of fluorophores enhance brightness of signals.
  • Improved localization accuracy ideal for super resolution imaging.
2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-mEOS antibody [1E8] - FluoTag®-X2 (AB325302)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-mEOS antibody [1E8] - FluoTag®-X2 (AB325302)

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf : non-fluorescent) were stained with ab325302 at a 1/500 dilution. (A) Greyscale image of the staining performed with ab325302. A typical mitochondrial staining was observed. (B) False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). (C) The corresponding DAPI signal of the depicted section. (D) Merge of all channels. False color representation of the DAPI signal : blue; the mEOS signal : magenta.

Other - Alexa Fluor® 647 Anti-mEOS antibody [1E8] - FluoTag®-X2 (AB325302)
  • Other

Supplier Data

Other - Alexa Fluor® 647 Anti-mEOS antibody [1E8] - FluoTag®-X2 (AB325302)

FluoTag®

FluoTag® reagents are alpaca-derived single-domain antibodies (sdAbs) directly conjugated to a defined number of fluorophores at known positions.

Depending on the specific target protein, they are available in up to three variants :
FluoTag®-Q : A single sdAb conjugated to a single fluorophore, ideal for quantitative readout.
FluoTag®-X2 : A single sdAb conjugated to two fluorophores, our standard variant.
FluoTag®-X4 : A blend of two different FluoTags®-X2 that bind to a single target. FluoTag®-X4 reagents effectively label the target (T) with 4 fluorophores in total, resulting in brighter signals.

关键信息

宿主种属

Alpaca

克隆

Monoclonal

克隆号

1E8

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

应用

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Lobophyllia hemprichii": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

产品详情

FluoTags®

FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.

Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.

FluoTag®-X2 (One target - One sdAb – Two fluorophores (1:1:2)

FluoTag®-X2 reagents are composed of a single sdAb carrying two fluorophores per protein molecule. The maximal displacement of fluorophores from the antigen-binding surface is ~3 nm. FluoTag®-X2 reagents show good tissue penetration features.

Features:

  • High affinity monovalent binding + two fluorophores per sdAb for brighter signals
  • Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能和储存信息

形式
Liquid
纯化工艺
Size-exclusion chromatography
存储溶液
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1 month
推荐的短期储存条件
-20°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
websiteProtocolBooklet
en

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com