重组Alexa Fluor® 647荧光Anti-MCP1抗体[EPR27464-89] (ab317532)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MCP1 antibody [EPR27464-89] (ab317532)](https://www.abcam.cn/ps/products/317/ab317532/Images/ab317532-2-alexa-fluor-647-anti-mcp1-antibody-epr27464-89-immunohistochemistry-image-amouse-lung.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MCP1 antibody [EPR27464-89] (ab317532)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR27464-89] to MCP1
- Suitable for: IHC-P
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 647荧光Anti-MCP1抗体[EPR27464-89]
参阅全部 MCP1 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR27464-89] to MCP1 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC-P: Mouse lung treated with 1µg/ml LPS and 1µg/ml BFA.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27464-89 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317532于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
-
功能
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 20296 Mouse
- SwissProt: P10148 Mouse
- Unigene: 290320 Mouse
-
别名
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MCP1 antibody [EPR27464-89] (ab317532)
Immunohistochemical analysis of paraffin-embedded Image A Mouse lung tissue labeling MCP1 with ab317532 at 1/100 (5.0 ug/ml) dilution.
Cytoplasmic staining on mouse lung treated with 1ug/ml Lipopolysaccharides (LPS) and 1ug/ml Brefeldin A for 16 hours (Image A); nearly no staining on untreated mouse lung (Image B).The section was incubated with ab317532 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MCP1 antibody [EPR27464-89] (ab317532)](https://www.abcam.cn/ps/products/317/ab317532/Images/ab317532-3-alexa-fluor-647-anti-mcp1-antibody-epr27464-89-immunohistochemistry-mouse-skeletal-muscl.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MCP1 antibody [EPR27464-89] (ab317532)Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling MCP1 with ab317532 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining on mouse skeletal muscle.The section was incubated with ab317532 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins
-
![Alexa Fluor® 647 Anti-MCP1 antibody [EPR27464-89] (ab317532)](https://www.abcam.cn/ps/products/317/ab317532/Images/ab317532-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-MCP1 antibody [EPR27464-89] (ab317532)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab317532 尚未被引用在任何文献中。