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AB303467

重组Alexa Fluor® 647荧光Anti-Ly6g抗体[EPR22909-135]

Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135]

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(2 Publications)

Rabbit Recombinant Monoclonal Ly6g antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

查看别名

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue stained for Ly6g using ab303467 at a 1/50 dilution (10.0 μg/ml) (Red). Positive staining on scattered neutrophil-like cells (arrows) in mouse spleen. The section was incubated with ab303467 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue stained for Ly6g using ab303467 at a 1/50 dilution (10.0 μg/ml) (Red). Positive staining on scattered cells (arrows) in mouse lung. The section was incubated with ab303467 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins).

Flow Cytometry - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)

Flow Cytometry analysis of mouse blood cells (Right panel) compared to Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Left panel) using ab303467 at a 1/50 dilution (1µg). Cells were stained with rabbit IgG or ab303467. Then stained with anti-CD11b conjugated to BV510. Gated on viable cells.

不同偶联物与剂型 (7)

  • Unconjugated

    Anti-Ly6g antibody [EPR22909-135]

  • Carrier free

    Anti-Ly6g antibody [EPR22909-135] - BSA and Azide free

  • 660 APC

    APC Anti-Ly6g antibody [EPR22909-135]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Ly6g antibody [EPR22909-135]

  • 519 FITC

    FITC Anti-Ly6g antibody [EPR22909-135]

  • 578 PE

    PE Anti-Ly6g antibody [EPR22909-135]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22909-135

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

反应种属

Mouse, Mouse

应用

IHC-P, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB25025

FITC Anti-Ly6c antibody [HK1.4]

  • 0
  • 0
Primary Antibodies

AB313900

Alexa Fluor® 488 Anti-CD11c antibody [KB90]

Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11c antibody [KB90] (AB313900)

  • 0
  • 0
Primary Antibodies

AB312914

Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

Recombinant

Flow Cytometry - Alexa Fluor® 594 Anti-CD11b antibody [M1/70] (AB312914)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Ly6g
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:39 PubMed40169936

2025

MAFF alleviates hepatic ischemia-reperfusion injury by regulating the CLCF1/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dengliang Lei,Yihua Wang,Shanshan Li,Song Xiang,Yunhai Luo,Ping Yan,Fang Luo,Zuotian Huang,ZhongJun Wu

BMC medical genomics 16:314 PubMed38049811

2023

Key mA regulators mediated methylation modification pattern and immune infiltration characterization in hepatic ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yixi Zhang,Can Qi,Yiwen Guo,Xuefeng Li,Zebin Zhu
View all publications
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