重组Alexa Fluor® 647荧光Anti-IL3RA/CD123抗体[EPR24481-20] (ab281333)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IL3RA/CD123 antibody [EPR24481-20] (ab281333)](https://www.abcam.cn/ps/products/281/ab281333/Images/ab281333-2-anti-IL3RA-CD123-antibody-EPR2448120-ICC-IF-THP1.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IL3RA/CD123 antibody [EPR24481-20] (ab281333)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR24481-20] to IL3RA/CD123
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-IL3RA/CD123抗体[EPR24481-20]
参阅全部 IL3RA/CD123 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR24481-20] to IL3RA/CD123 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: THP-1 cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24481-20 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281333于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
This product gave a positive signal in THP-1 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. This product gave a positive signal in THP-1 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
靶标
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功能
This is a receptor for interleukin-3. -
序列相似性
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3563 Human
- Omim: 308385 Human
- Omim: 430000 Human
- SwissProt: P26951 Human
- Unigene: 632790 Human
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别名
- CD123 antibody
- CD123 antigen antibody
- hIL 3Ra antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IL3RA/CD123 antibody [EPR24481-20] (ab281333)
ab281333 staining IL3RA/CD123 in THP-1 cells, with negative expression in K562 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab281333 at 5 µg/ml (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2 µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in THP-1 cells fixed with 100% methanol (5 min).
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![Alexa Fluor® 647 Anti-IL3RA/CD123 antibody [EPR24481-20] (ab281333)](https://www.abcam.cn/ps/products/281/ab281333/Images/ab281333-456199-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 647 Anti-IL3RA/CD123 antibody [EPR24481-20] (ab281333)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab281333 尚未被引用在任何文献中。