AB199215

重组Alexa Fluor® 647荧光Anti-IL-2抗体[EPR2780]

Alexa Fluor® 647 Anti-IL-2 antibody [EPR2780]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal IL-2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications.

查看别名

Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IL-2 antibody [EPR2780] (AB199215)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IL-2 antibody [EPR2780] (AB199215)

ab199215 staining IL2 in Jurkat cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199215 at 1/50 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

不同偶联物与剂型 (1)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-IL-2 antibody [EPR2780]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2780

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

反应种属

Human

应用

ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed : 6438535). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) (PubMed : 16293754, PubMed : 16477002). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3 (PubMed : 7973659). In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed : 8580378). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways (PubMed : 25142963). Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well (PubMed : 6608729). Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production (PubMed : 6438535). Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.

See full target information IL2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Pediatrics international : official journal of the Japan Pediatric Society 64:e15159 PubMed35727889

2022

The histone deacetylase inhibitor OBP-801 has in vitro/in vivo anti-neuroblastoma activity.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Kaneda,Tomoko Iehara,Ken Kikuchi,Yohei Sugimoto,Norio Nakagawa,Shigeki Yagyu,Mitsuru Miyachi,Eiichi Konishi,Toshiyuki Sakai,Hajime Hosoi

Clinica chimica acta; international journal of cli 487:256-263 PubMed30292629

2018

Establishment of a protein biochip to detect serum IgG antibodies against IL-2 and soluble CD25 in hemophagocytic lymphohistiocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Sheng-Sheng Liu,Song-Guo Li,Yi Gao,Lei Ye,Gabrielle Olivia Ramsay Johnson,Zi-Jian Song,Wei-Dong Du

View all publications

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重组Alexa Fluor® 647荧光Anti-IL-2抗体[EPR2780]