重组Alexa Fluor® 647荧光Anti-Histone H2B (mutated E76Q)抗体[EPR27299-85] (ab317717)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)](https://www.abcam.cn/ps/products/317/ab317717/Images/ab317717-3-alexa-fluor-647-anti-histone-h2b-mutated-e76q-antibody-epr27299-85-immunohistochemistry-a-hek-293t-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR27299-85] to Histone H2B (mutated E76Q)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-Histone H2B (mutated E76Q)抗体[EPR27299-85]
参阅全部 Histone H2B 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR27299-85] to Histone H2B (mutated E76Q) -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF:293T cells transfected with a human H2BC4 E76Q expression vector. IHC-P: HEK-293T transfected with a human H2BC4 E76Q expression vector containing a His-tag cell pellet.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27299-85 -
同种型
IgG
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317717于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/250.
|
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
1/250. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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相关性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
- SwissProt: P33778 Human
- SwissProt: P57053 Human
see all -
别名
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Histone H2B (mutated E76Q) with ab317717 at 1/100 (5.0 ug/ml) dilution.
Positive staining on (B) HEK-293T transfected with a human H2BC4 E76Q expression vector containing a His-tag cell pellet. No staining on (A) HEK-293T transfected with a human H2BC4 (WT) expression vector containing a His-tag cell pellet and (C) HEK-293T transfected with an empty vector containing a His-tag cell pellet.The section was incubated with ab317717 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)](https://www.abcam.cn/ps/products/317/ab317717/Images/ab317717-4-alexa-fluor-647-anti-histone-h2b-mutated-e76q-antibody-epr27299-85-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Histone H2B (mutated E76Q) with ab317717 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining on human cerebrum. The section was incubated with ab317717 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)](https://www.abcam.cn/ps/products/317/ab317717/Images/ab317717-5-alexa-fluor-647-anti-histone-h2b-mutated-e76q-antibody-epr27299-85-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Histone H2B (mutated E76Q) with ab317717 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining on mouse cerebrum. The section was incubated with ab317717 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)](https://www.abcam.cn/ps/products/317/ab317717/Images/ab317717-6-alexa-fluor-647-anti-histone-h2b-mutated-e76q-antibody-epr27299-85-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Histone H2B (mutated E76Q) with ab317717 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining on rat cerebrum. The section was incubated with ab317717 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)](https://www.abcam.cn/ps/products/317/ab317717/Images/ab317717-2-alexa-fluor-647-anti-histone-h2b-mutated-e76q-antibody-epr27299-85-immunocytochemistry-293t.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Histone H2B (mutated E76Q) with ab317717 at 1/250 (2.0 ug/ml) dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a human H2BC4 E76Q expression vector containing a myc tag, while showing no staining in 293T cells transfected with a human H2BC4 (WT) expression vector containing a myc tag(shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 0.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
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![Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)](https://www.abcam.cn/ps/products/317/ab317717/Images/ab317717-1-BenefitsofRecombinantantibodies.jpg)
Alexa Fluor® 647 Anti-Histone H2B (mutated E76Q) antibody [EPR27299-85] (ab317717)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317717 尚未被引用在任何文献中。