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AB237385

重组Alexa Fluor® 647荧光Anti-EpCAM抗体[EPR20533-63]

Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63]

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(2 Publications)

Rabbit Recombinant Monoclonal EPCAM antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 2 publications.

查看别名

CD326, Tacstd1, Epcam, Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, Protein 289A, Tumor-associated calcium signal transducer 1, EGP314, mEGP314

4 Images
Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)

Flow cytometric analysis of unfixed 4T1 (right) and NIH/3T3 (left) cells with ab237385 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237385, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 17mW red Helium-Neon laser (633nm) and 660/20 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)

ab237385 staining EpCAM in 4T1 cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.

The cells were then incubated overnight at +4°C with ab237385 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)

ab237385 showing no staining of EpCAM in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.

The cells were then incubated overnight at +4°C with ab237385 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)

Flow cytometry overlay histogram showing M158 positive cells (left) and negative NIH/3T3 cells (right) stained with ab237385 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab237385) (1x 106 in 100 μl at 0.1 μg/ml (1/5000)) for 30 min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

不同偶联物与剂型 (4)

  • Unconjugated

    Anti-EpCAM antibody [EPR20533-63]

  • Carrier free

    Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-EpCAM antibody [EPR20533-63]

  • 578 PE

    PE Anti-EpCAM antibody [EPR20533-63]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20533-63

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

No

反应种属

Mouse

应用

Flow Cyt, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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替代产品

Primary Antibodies

AB237397

PE Anti-EpCAM antibody [EPR20532-225]

RabMAb|Recombinant

Immunohistochemistry paraffin embedded sections - PE Anti-EpCAM antibody [EPR20532-225] (AB237397)

  • 0
  • 0
Primary Antibodies

AB237398

APC Anti-EpCAM antibody [EPR20532-225]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - APC Anti-EpCAM antibody [EPR20532-225] (AB237398)

  • 0
  • 0
Primary Antibodies

AB124825

Anti-EpCAM antibody [EPR677(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

  • 1
  • 1
Primary Antibodies

AB225894

Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free (AB225894)

  • 0
  • 0
Primary Antibodies

AB213501

Anti-EpCAM antibody [EPR20533-266]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] (AB213501)

  • 0
  • 0
Primary Antibodies

AB32392

Anti-EpCAM antibody [E144]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-EpCAM antibody [E144] (AB32392)

  • 4
  • 19
Primary Antibodies

AB223582

Anti-EpCAM antibody [EPR20532-225]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] (AB223582)

  • 5
  • 2

搭配使用产品

Assay Kits

AB264632

Human EpCAM ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human EpCAM ELISA Kit (AB264632)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB193895

Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] (AB193895)

  • 5
  • 4
Primary Antibodies

AB252149

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

  • 0
  • 0
Primary Antibodies

AB303477

Alexa Fluor® 647 Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 647 Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38] (AB303477)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/5000", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in 4T1 cells fixed with 100% methanol (5 min)</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
See full target information Epcam
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 13:e70264 PubMed40051209

2025

Acute tuft cell ablation in mice induces malabsorption and alterations in secretory and immune cell lineages in the small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Momoh,Francisca Adeniran,Cynthia Ramos,Kathleen E DelGiorno,Hiroshi Seno,Joseph T Roland,Izumi Kaji

Nature communications 12:289 PubMed33436554

2021

Nanoparticle delivery of microRNA-146a regulates mechanotransduction in lung macrophages and mitigates injury during mechanical ventilation.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher M Bobba,Qinqin Fei,Vasudha Shukla,Hyunwook Lee,Pragi Patel,Rachel K Putman,Carleen Spitzer,MuChun Tsai,Mark D Wewers,Robert J Lee,John W Christman,Megan N Ballinger,Samir N Ghadiali,Joshua A Englert
View all publications
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