AB310289

重组Alexa Fluor® 647荧光Anti-DNA Polymerase beta抗体[EPR12722(B)]

Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal DNA Polymerase beta antibody - conjugated to Alexa Fluor® 647.

查看别名

DNA polymerase beta, 5'-deoxyribose-phosphate lyase, AP lyase, 5'-dRP lyase, POLB

不同偶联物与剂型 (8)

578 PE

PE Anti-DNA Polymerase beta antibody [EPR12722(B)]
660 APC

APC Anti-DNA Polymerase beta antibody [EPR12722(B)]
HRP

HRP Anti-DNA Polymerase beta antibody [EPR12722(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-DNA Polymerase beta antibody [EPR12722(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-DNA Polymerase beta antibody [EPR12722(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-DNA Polymerase beta antibody [EPR12722(B)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-DNA Polymerase beta antibody [EPR12722(B)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-DNA Polymerase beta antibody [EPR12722(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12722(B)

亚型

IgG

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

不含载体蛋白

应用

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The human recommendation is based on the WB results. We do not guarantee IHC-P for human

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产品详情

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DNA polymerase beta often abbreviated as pol β serves as a repair enzyme that plays a role in the base excision repair (BER) pathway. This protein's mechanical function involves filling small gaps in the DNA created during repair processes by adding nucleotides. Its molecular weight is approximately 39 kDa. DNA polymerase beta is expressed extensively in the brain liver and testis indicating its importance in DNA repair in various cells. Other names for this protein include DNA pol beta and pol β. It synthesizes DNA using deoxyribonucleotide triphosphates as substrates and requires magnesium ions for activity.

Biological function summary

DNA polymerase beta helps to maintain genome integrity by repairing DNA lesions caused by oxidation alkylation or deamination. This protein often collaborates with other components of the DNA repair pathways but it does not directly form a larger protein complex focusing instead on its important function in BER. When DNA damage like single-strand breaks occur pol β carries out important gap-filling synthesis steps preparing the strand for subsequent ligation.

Pathways

DNA polymerase beta functions critically in base excision repair and acts alongside other important proteins like XRCC1. The BER pathway operates to correct DNA damage from endogenous sources preventing harmful mutations. This pathway is essential for maintaining cellular stability and protecting cells from apoptosis or malignant transformation that might occur if DNA damage propagates.

Mutations or dysfunctional activity within DNA polymerase beta have links to cancer and neurodegenerative diseases. For example errors in pol β's repair functions can lead to the persistence of DNA damage which can contribute to tumorigenesis. Furthermore its relationship with beta-actin has implications in cellular structural integrity and cancer metastasis. Dysfunctions within the BER pathway indicate potential contributions to neurodegenerative disorders where an accumulation of DNA damage is a common feature.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Repair polymerase that plays a key role in base-excision repair (PubMed : 10556592, PubMed : 9207062, PubMed : 9572863). During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap (PubMed : 10556592, PubMed : 17526740, PubMed : 9556598, PubMed : 9572863, PubMed : 9614142). Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase (PubMed : 9614142).

See full target information POLB

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Alexa Fluor® 647荧光Anti-DNA Polymerase beta抗体[EPR12722(B)]