重组Alexa Fluor® 647荧光Anti-Dectin-1抗体[EPR28032-27] (ab318175)
![Flow Cytometry - Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] (ab318175)](https://www.abcam.cn/ps/products/318/ab318175/Images/ab318175-1-alexa-fluor-647-anti-dectin-1-antibody-epr28032-27-flow-cytometry-human-pbmc.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] (ab318175)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR28032-27] to Dectin-1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-Dectin-1抗体[EPR28032-27]
参阅全部 Dectin-1 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR28032-27] to Dectin-1 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Human PBMC cell.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28032-27 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318175于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/5000.
|
| 说明 |
|---|
|
Flow Cyt
1/5000. |
靶标
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功能
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. -
组织特异性
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta. -
疾病相关
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
序列相似性
Contains 1 C-type lectin domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to glucan binding. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 64581 Human
- Omim: 606264 Human
- SwissProt: Q9BXN2 Human
- Unigene: 143929 Human
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别名
- Beta GR antibody
- Beta-glucan receptor antibody
- BGR antibody
see all
图片
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Flow Cytometry - Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] (ab318175)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Dectin-1 with ab318175 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Cells are co-stained with CD14 conjugated to Alexa Fluor® 488. -
![Flow Cytometry - Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] (ab318175)](https://www.abcam.cn/ps/products/318/ab318175/Images/ab318175-2-alexa-fluor-647-anti-dectin-1-antibody-epr28032-27-flow-cytometry-human-pbmc1.jpg)
Flow Cytometry - Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] (ab318175)Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Dectin-1 with ab318175 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Gated on viable cells.
Cells are co-stained with CD3 conjugated to Alexa Fluor® 488. -
![Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] (ab318175)](https://www.abcam.cn/ps/products/318/ab318175/Images/ab318175-3-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 647 Anti-Dectin-1 antibody [EPR28032-27] (ab318175)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab318175 尚未被引用在任何文献中。