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重组RabMAb

重组Alexa Fluor® 647荧光Anti-COL1A2抗体[EPR24262-52] (ab281330)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-COL1A2 antibody [EPR24262-52] (ab281330)
  • Alexa Fluor® 647 Anti-COL1A2 antibody [EPR24262-52] (ab281330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR24262-52] to COL1A2
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 750 APC PE Unconjugated

概述

  • 产品名称

    Alexa Fluor® 647荧光Anti-COL1A2抗体[EPR24262-52]
    参阅全部 COL1A2 一抗

  • 描述

    Alexa Fluor® 647荧光兔单克隆抗体[EPR24262-52] to COL1A2

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC: human normal colon tissue sections

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281330于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Type I collagen is a member of group I collagen (fibrillar forming collagen).

  • 组织特异性

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

  • 疾病相关

    Ehlers-Danlos syndrome 7B
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.

  • 序列相似性

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.

  • 结构域

    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

  • 翻译后修饰

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Alpha 2 collagen type I antibody
      • Alpha 2 type I collagen antibody
      • Alpha 2 type I procollagen antibody
      • Alpha 2(I) collagen antibody
      • Alpha 2(I) procollagen antibody
      • Alpha-2 type I collagen antibody
      • CO1A2_HUMAN antibody
      • COL1A2 antibody
      • Collagen alpha 2(I) chain antibody
      • Collagen alpha-2(I) chain antibody
      • Collagen I alpha 2 polypeptide antibody
      • Collagen of skin tendon and bone alpha 2 chain antibody
      • Collagen type I alpha 2 antibody
      • OI4 antibody
      • Osteogenesis imperfecta type IV antibody
      • Type I procollagen antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-COL1A2 antibody [EPR24262-52] (ab281330)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-COL1A2 antibody [EPR24262-52] (ab281330)

      Immunofluorescence staining of COL1A2 staining in a section of formalin-fixed paraffin-embedded human normal colon* tissue.

      The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab281330 at 1/500 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Alexa Fluor® 647 Anti-COL1A2 antibody [EPR24262-52] (ab281330)
      Alexa Fluor® 647 Anti-COL1A2 antibody [EPR24262-52] (ab281330)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab281330？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab281330 尚未被引用在任何文献中。

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