重组Alexa Fluor® 647荧光Anti-CDCA5抗体[EPR16331] (ab225376)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CDCA5 antibody [EPR16331] (ab225376)](https://www.abcam.cn/ps/products/225/ab225376/Images/ab225376-2-antiCDCA5ab225376Flow-CytometryHumanMCF7-Cells.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CDCA5 antibody [EPR16331] (ab225376)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR16331] to CDCA5
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-CDCA5抗体[EPR16331]
参阅全部 CDCA5 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR16331] to CDCA5 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: Flow Cyt (Intra)more details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): MCF7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.02% PBS, 0.01% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16331 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
- PE Anti-CDCA5 antibody [EPR16331] - C-terminal (ab225377)
- Anti-CDCA5 antibody [EPR16331] - BSA and Azide free (ab240328)
- APC Anti-CDCA5 antibody [EPR16331] – C–terminal (ab319299)
- Alexa Fluor® 488 Anti-CDCA5 antibody [EPR16331] – C–terminal (ab319573)
- Alexa Fluor® 594 Anti-CDCA5 antibody [EPR16331] – C–terminal (ab319792)
- Alexa Fluor® 555 Anti-CDCA5 antibody [EPR16331] – C–terminal (ab319933)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225376于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/5000. |
靶标
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功能
Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair. -
序列相似性
Belongs to the sororin family. -
结构域
The KEN box is required for the association with the APC/C complex. -
翻译后修饰
Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
Ubiquitinated by the APC/C complex in G1, leading to its degradation. -
细胞定位
Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown. - Information by UniProt
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数据库链接
- Entrez Gene: 113130 Human
- Omim: 609374 Human
- SwissProt: Q96FF9 Human
- Unigene: 434886 Human
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别名
- CDCA5 antibody
- CDCA5_HUMAN antibody
- Cell division cycle associated protein 5 antibody
see all
图片
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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CDCA5 antibody [EPR16331] (ab225376)
Overlay histogram showing MCF7 cells stained with ab225376 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS/10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab225376) (1x106 in 100 µl at 0.1 µg/ml (1/5000 dilution)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Alexa Fluor® 647 Anti-CDCA5 antibody [EPR16331] (ab225376)](https://www.abcam.cn/ps/products/225/ab225376/Images/ab225376-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-CDCA5 antibody [EPR16331] (ab225376)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225376 尚未被引用在任何文献中。