重组Alexa Fluor® 647荧光Anti-CCR4抗体[EPR23502-85] (ab281322)
![Flow Cytometry - Alexa Fluor® 647 Anti-CCR4 antibody [EPR23502-85] (ab281322)](https://www.abcam.cn/ps/products/281/ab281322/Images/ab281322-484998-anti-ccr4-human-pbmc-flow-cytometry.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-CCR4 antibody [EPR23502-85] (ab281322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR23502-85] to CCR4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 647荧光Anti-CCR4抗体[EPR23502-85]
参阅全部 CCR4 一抗 -
描述
Alexa Fluor® 647荧光兔单克隆抗体[EPR23502-85] to CCR4 -
宿主
Rabbit -
偶联物
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: Human peripheral blood mononuclear cells (PBMCs).
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23502-85 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/100.
|
| 说明 |
|---|
|
Flow Cyt
1/100. |
靶标
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功能
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
组织特异性
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1233 Human
- GenBank: NP_005499.1 Human
- Omim: 604836 Human
- SwissProt: P51679 Human
- Unigene: 184926 Human
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别名
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
图片
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Flow Cytometry - Alexa Fluor® 647 Anti-CCR4 antibody [EPR23502-85] (ab281322)
Flow cytometric staining of human peripheral blood mononuclear cells (PBMCs) with ab281322 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 647 isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab281322) or Rabbit IgG (monoclonal) Alexa Fluor® 647 isotype (100 μl at 5 μg/ml (1/100)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on live lymphocytes.
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![Alexa Fluor® 647 Anti-CCR4 antibody [EPR23502-85] (ab281322)](https://www.abcam.cn/ps/products/281/ab281322/Images/ab281322-456180-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 647 Anti-CCR4 antibody [EPR23502-85] (ab281322)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab281322 尚未被引用在任何文献中。