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使用敲除细胞株进行验证重组RabMAb

重组Alexa Fluor® 647荧光Anti-Calnexin抗体[EPR3632] (ab225062)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)
  • Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR3632] to Calnexin
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 750 APC PE Unconjugated

概述

  • 产品名称

    Alexa Fluor® 647荧光Anti-Calnexin抗体[EPR3632]
    参阅全部 Calnexin 一抗

  • 描述

    Alexa Fluor® 647荧光兔单克隆抗体[EPR3632] to Calnexin

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • 特异性

    Recognizes ER membrane, mitochondria and cis-Golgi.

  • 经测试应用

    适用于: ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: HeLa and wildtype HAP1 cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR3632

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225062于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/500.

This product gave a positive signal in HeLa cells and wildtype HAP1 cells fixed with 100% methanol (5 min)
说明
ICC/IF
1/100 - 1/500.

This product gave a positive signal in HeLa cells and wildtype HAP1 cells fixed with 100% methanol (5 min)

靶标

  • 功能

    Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

  • 序列相似性

    Belongs to the calreticulin family.

  • 细胞定位

    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Calnexin antibody
      • CALX_HUMAN antibody
      • CANX antibody
      • CNX antibody
      • FLJ26570 antibody
      • Histocompatibility complex class I antigen binding protein p88 antibody
      • IP90 antibody
      • Major histocompatibility complex class I antigen-binding protein p88 antibody
      • p90 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)

      ab225062 staining Calnexin in wild-type HAP1 cells and knockout cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab225062 at 1μg/ml (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)

      ab225062 staining Calnexin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225062 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)
      Alexa Fluor® 647 Anti-Calnexin antibody [EPR3632] (ab225062)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab225062？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab225062 被引用在 1 文献中.

    • Zábranská H  et al. Biogenesis of hepatitis B virus e antigen is driven by translocon-associated protein complex and regulated by conserved cysteine residues within its signal peptide sequence. FEBS J 289:2895-2914 (2022). PubMed: 34839586

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