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重组RabMAb

重组Alexa Fluor® 647荧光Anti-Aryl hydrocarbon Receptor抗体[EPR7119(N)(2)] (ab225358)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab225358)
  • Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab225358)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR7119(N)(2)] to Aryl hydrocarbon Receptor
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 APC PE Unconjugated

概述

  • 产品名称

    Alexa Fluor® 647荧光Anti-Aryl hydrocarbon Receptor抗体[EPR7119(N)(2)]
    参阅全部 Aryl hydrocarbon Receptor 一抗

  • 描述

    Alexa Fluor® 647荧光兔单克隆抗体[EPR7119(N)(2)] to Aryl hydrocarbon Receptor

  • 宿主

    Rabbit

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • 经测试应用

    适用于: ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: HeLa cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225358于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)
说明
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

靶标

  • 功能

    Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.

  • 组织特异性

    Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.

  • 序列相似性

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • 细胞定位

    Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Ah receptor antibody
      • AhR antibody
      • AHR_HUMAN antibody
      • Aromatic hydrocarbon receptor antibody
      • Aryl hydrocarbon receptor antibody
      • Aryl hydrocarbon receptor precursor antibody
      • bHLHe76 antibody
      • Class E basic helix loop helix protein 76 antibody
      • Class E basic helix-loop-helix protein 76 antibody
      • HGNC:348 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab225358)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab225358)

      ab225358 staining Aryl hydrocarbon Receptor in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225358 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab225358)
      Alexa Fluor® 647 Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab225358)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab225358？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab225358 被引用在 1 文献中.

    • Gao H  et al. Benzo[a]pyrene at human blood equivalent level induces human lung epithelial cell invasion and migration via aryl hydrocarbon receptor signaling. J Appl Toxicol 40:1087-1098 (2020). PubMed: 32166782

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