重组Alexa Fluor® 594荧光Anti-CD209B (DC-SIGNR1)抗体[EPR27427-87] (ab317363)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)](https://www.abcam.cn/ps/products/317/ab317363/Images/ab317363-1-alexa-fluor-594-anti-cd209-antibody-epr27427-87-immunohistochemistry-mouse-spleen.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR27427-87] to CD209B/DC-SIGNR1
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 594荧光Anti-CD209B (DC-SIGNR1)抗体[EPR27427-87]
参阅全部 CD209B/DC-SIGNR1 一抗 -
描述
Alexa Fluor® 594荧光兔单克隆抗体[EPR27427-87] to CD209B/DC-SIGNR1 -
宿主
Rabbit -
偶联物
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Mouse spleen and Rat spleen tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27427-87 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317363于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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相关性
SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides. -
细胞定位
Single-pass type II membrane protein -
数据库链接
- Entrez Gene: 69165 Mouse
- Entrez Gene: 288378 Rat
- SwissProt: Q8CJ91 Mouse
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别名
- CD209 antigen like protein B antibody
- CD209b antigen antibody
- DC SIGN related protein 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD209B/DC-SIGNR1 with AB317363 at 1/100 (5.0 ug/ml) dilution.
Positive staining on mouse spleen. The section was incubated with ab317363 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)](https://www.abcam.cn/ps/products/317/ab317363/Images/ab317363-2-alexa-fluor-594-anti-cd209-antibody-epr27427-87-immunohistochemistry-rat-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD209B/DC-SIGNR1 with AB317363 at 1/100 (5.0 ug/ml) dilution.
Positive staining on rat spleen. The section was incubated with ab317363 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)](https://www.abcam.cn/ps/products/317/ab317363/Images/ab317363-4-alexa-fluor-594-anti-cd209-antibody-epr27427-87-immunohistochemistry-rat-skeletal-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling CD209B/DC-SIGNR1 with AB317363 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining on rat skeletal muscle. The section was incubated with ab317363 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)](https://www.abcam.cn/ps/products/317/ab317363/Images/ab317363-3-alexa-fluor-594-anti-cd209-antibody-epr27427-87-immunohistochemistry-mouse-skeletal-muscl.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CD209B/DC-SIGNR1 with AB317363 at 1/100 (5.0 ug/ml) dilution.
Negative control: no staining on mouse skeletal muscle. The section was incubated with ab317363 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Counterstained with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)](https://www.abcam.cn/ps/products/317/ab317363/Images/ab317363-5-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 594 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (ab317363)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317363 尚未被引用在任何文献中。