AB216385

重组Alexa Fluor® 594荧光Anti-ATP5A抗体[EPR13030(B)]

Name: Alexa Fluor® 594 Anti-ATP5A抗体[EPR13030(B)] - Mitochondrial Marker (ab216385)| Abcam中文官网
Brand: Abcam Limited
SKU: AB216385
Price: 5206 CNY
Availability: InStock
Rating: 4 (1 reviews)

Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

RabMAb
Recombinant
了解详情

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal ATP5A antibody - conjugated to Alexa Fluor® 594. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

查看别名

ATP5A, ATP5A1, ATP5AL2, ATPM, ATP5F1A, ATP synthase F1 subunit alpha

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB216385)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB216385)

ab216385 staining ATP5A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab216385 at 1/1000 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

不同偶联物与剂型 (5)

Unconjugated

Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker
Carrier free

Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker
HRP

HRP Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13030(B)

亚型

IgG

偶联物

Alexa Fluor® 594

激发波长/发射波长

Ex: 590nm, Em: 617nm

不含载体蛋白

反应种属

Human, Human, Mouse, Rat

应用

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ATP5A also known as ATP synthase F1 subunit alpha is a protein important for cellular energy production. As part of the ATP synthase complex it plays a mechanical role in synthesizing ATP from ADP and inorganic phosphate. The ATP5A protein has a molecular weight of approximately 55 kDa and is widely expressed in the inner mitochondrial membrane across different cell types. Its central function lies in its ability to harness the energy of the proton gradient generated by the electron transport chain to catalyze ATP synthesis.

Biological function summary

ATP5A is essential in cellular respiration serving as a catalytic core of the F1 component of ATP synthase. As part of the multi-subunit enzyme complex ATP synthase is responsible for ATP production the primary energy currency in cells. The ATP5A subunit works in conjunction with other subunits of the enzyme oligomer to facilitate the conversion of energy released during oxidative phosphorylation into a usable form. The protein's efficiency in this biological role underpins its importance in sustaining cellular energy homeostasis.

Pathways

ATP5A plays a pivotal role in oxidative phosphorylation and the electron transport chain integral components of cellular respiration. The oxidative phosphorylation pathway depends on this protein to manage the synthesis of ATP molecules while the electron transport chain creates the proton gradient necessary for ATP production. ATP5A is functionally connected to other proteins in these pathways such as ATP5B and cytochrome c oxidase working in a coordinated manner to ensure efficient energy transfer and maintenance.

ATP5A is implicative in mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome and Parkinson's disease. These conditions often arise from deficits in ATP production where ineffective ATP synthase activity can contribute to cellular energy failures. In the context of Parkinson’s disease for instance ATP5A interactions with other proteins like Parkin can contribute to mitochondrial dysfunction an important pathological feature of the disorder. Through such associations alterations in ATP5A activity can significantly impact disease progression and symptomatology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the catalytic subunit beta (ATP5F1B), forms the catalytic core in the F(1) domain (PubMed : 37244256). Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (Probable). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed : 30146159).

See full target information ATP5F1A

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7272 PubMed36433992

2022

Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Scur,Ahmad Bakur Mahmoud,Sayanti Dey,Farah Abdalbarri,Iona Stylianides,Daniel Medina-Luna,Gayani S Gamage,Aaron Woblistin,Alexa N M Wilson,Haggag S Zein,Ashley Stueck,Andrew Wight,Oscar A Aguilar,Francesca Di Cara,Brendon D Parsons,Mir Munir A Rahim,James R Carlyle,Andrew P Makrigiannis

Cell death & disease 13:324 PubMed35395834

2022

Suppression of histone deacetylase 1 by JSL-1 attenuates the progression and metastasis of cholangiocarcinoma via the TPX2/Snail axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Xu,Weizhong Yang,Jinhui Che,Deqiang Li,Haihong Wang,Yunjiu Li,Wuyuan Zhou

Communications biology 3:305 PubMed32533056

2020

Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia J Ahl,Richard A Hopkins,Wen Wei Xiang,Bijin Au,Nivashini Kaliaperumal,Anna-Marie Fairhurst,John E Connolly

View all publications

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重组Alexa Fluor® 594荧光Anti-ATP5A抗体[EPR13030(B)]