Alexa Fluor® 594荧光Anti-alpha Tubulin抗体[DM1A] - Microtubule Marker

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized FUS KO HAP1 (FUS knockout human chronic myelogenous leukemia near-haploid cell) cells labelling TLS/FUS with ab323832 at 1/1000 (1.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in parental HAP1 cell line (shown in green) and no staining in FUS KO HAP1 cells. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling GRP94 with ab314591 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling ER81/ETV1 with ab314874 at 1/50 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear staining in PC-3 cells. Negative control : MCF7(PMID : 27604655). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling ZC3H13 with ab314638 at 1/50 (9.98 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1009 (2ug/ml) dilution (Green). Confocal image showing nuclear staining in SW480 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1009 (2ug/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling Progesterone Receptor with ab313737 at 1/50 (10 ug/ml) dilution(Green). Confocal image showing nuclear staining in T47D cells. Negative control : HepG2 (PMID : 15297426). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Doublecortin with ab305266 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in SH-SY5Y cells. Negative control : HeLa (PMID : 18312642). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT (human thyroid carcinoma epithelial cell) cells labelling CGRP-I+CGRP-II with ab305115 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in TT cells. Negative control : 293T (PMID : 32296769). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling ITM2B with ab314654 at 1/100 (5.12 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1012 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1012 (2ug/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling USP48 with ab314543 at 1/50 (10.44 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing nuclear staining in 293T cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Histone H3 (phospho T3) with ab305218 at 1/500 (1 ug/ml) dilution. Confocal image showing nuclear staining in HeLa cells in M phase. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling alpha Tubulin with ab305219 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in HeLa cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling TIE2 with ab307374 at 1/100 (5.0 μg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in HUVEC cells. Negative control : HEK-293T (PMID : 17189382). ab195889 was used to counterstain tubulin at 1/200 (2.5μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling CCR9 with ab308445 at 1/50 (10.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing mainly membranous staining in MOTL-4 cell line, no staining was observed in HEK-293 cell line.Low expression : HEK-293 (PMID : 24870448). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling TLS/FUS with ab309787 at 1/50 (10.0 ug/ml) dilution (Green).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing nuclear staining in HepG2 cell line.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labelling SOX10 with ab309657 at 1/50 (10.0 ug/ml) dilution (Green).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing nuclear and weak cytoplasmic staining in A375 cells.

Negative control : HeLa (PMID 29315345)

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Ndufs4 with ab309701 at 1/50 (10.0 µg/ml) dilution.

Confocal image showing cytoplasmic staining in HeLa cell line (shown in green).
The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MAP2K1 KO HAP1 (MAP2K1 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling MEK1 (phospho T286) with ab307509 at 1/1000 (0.48 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal showing cytoplasmic and nuclear staining in wildtype HAP1 cells in M phase and no staining in MAP2K1 knockout HAP1 cells. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PTRF KO HeLa (PTRF knockout human cervical adenocarcinoma epithelial cel), ab265435 cells labelling PTRF with ab308623 at 1/500 (1.008 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in wildtype HeLa cells and showing no staining in PTRF knockout HeLa cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling Soluble TNF Receptor I + Fc Fragment with ab308506 at 1/50 (10.52 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in A549 cell line.Negative control : Raji (PMID : 10583368, PMID : 8144934). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling EXOSC10/RRP6 with ab324365 at 1/50 dilution (Green).

Confocal image showing nucleolar staining in Hela cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Hsp70 with ab307398 at 1/50 dilution (10 ug/ml) (Green). Confocal image showing cytoplasmic and nuclear staining in HeLa cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

ab196384 staining Cardiac Troponin I in A673 (Human muscle Ewing's Sarcoma cell line) cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196384 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human peripheral blood mononuclear cell) cells labeling VSIG4 with ab307773 at 1/50 dilution (10 µg/ml) (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC. Negative control : Jurkat (PMID : 16009265). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-204 (human muscle rhabdomyosarcoma cell) cells labeling PDGFR alpha with ab307855 at 1/50 dilution (10.0 ug/ml) (Green). Confocal image showing membranous and cytoplasmic staining in A-204 cells. Negative control : A-172 (PMID : 8425771). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling beta Catenin with ab305112 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in A431 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HDLM-2 (human Hodgkin lymphoma cell) cells labeling IL-2 Receptor with ab307865 at 1/50 dilution (10.0 ug/ml) (Green). Confocal image showing cytoplasmic staining in HDLM-2 cells. Negative control : PC-3 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Green). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labeling CTCF with ab300639 at 1/2000 dilution (0.266 μg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488, green) preadsorbed at 1 : 1000 (2 μg/mL). Confocal image showing nuclear staining in HeLa cell line.

ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (red) was used as a counterstain at 1 : 200 dilution (2.5 μg/ml). Nuclear counter satin is DAPI.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Glypican 3 with ab314880 at 1/50 (10.0 ug/ml) dilution. Confocal image showing membranous staining in HepG2 cells. Negative control : A431 (PMID : 25758784) It is the raw material of conjugates and worked well in ICC. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling TLS/FUS with ab309787 at 1/50 (10.0 ug/ml) dilution (Green).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing nuclear staining in K-562 cell line.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Rb with ab300671 at 1/50 dilution (10.0 µg/mL) (Green). Confocal image showing nuclear staining in MCF7 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling SUZ12 with ab307891 at 1/50 dilution (9.86 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/mL) (Green). Confocal image showing nuclear staining in HeLa cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Clathrin heavy chain with ab305259 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing punctuated cytoplasmic staining in HeLa cell line.The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with ab305363 at 1/50 dilution (10.0 ug/ml) (Green). Confocal image showing nucleolar staining in HeLa cells.

Negative control : MCF7 (PMID : 29904067).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized DROSHA KO HEK293T (DROSHA konckout human embryonic kidney cell line) cells labelling Drosha with ab315100 at 1/500 (1.046 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing nuclear staining in HEK293T cell line, and no staining in DROSHA KO HEK293T cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labeling EGFR with ab307848 at 1/50 dilution (10.0 µg/ml). Confocal image showing membranous and weak cytoplasmic staining in A431 cells. Negative control : Jurkat (PMID : 24045666). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Ndufs4 with ab309701 at 1/50 (10.0 µg/ml) dilution.

Confocal image showing cytoplasmic staining in HepG2 cell line (shown in green).
The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling GWL with ab307955 at 1/50 dilution (10.18 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing positive staining in HeLa cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml) (Green).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Histone H3 (phospho S10 + S28) with ab303567 at 1/4000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa in M phase. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

ab109028 staining HP1 alpha in HeLa (human cervical adenocarcinoma epithelial cell). The cells were fixed with 100% methanol, permeabilized with 0.1% TritonX-100. The cells were then incubated with ab109028 at 1 : 100 dilution. Cells were then incubated with ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1 : 1000 dilution (shown in green). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as counterstain at 1 : 200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Confocal image showing nuclear staining in HeLa cell line.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling eIF4A2 with ab307728 at 1/50 (9.92 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.