Alexa Fluor® 594荧光Anti-alpha Tubulin抗体[DM1A] - Microtubule Marker

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized FUS KO HAP1 (FUS knockout human chronic myelogenous leukemia near-haploid cell) cells labelling TLS/FUS with ab323832 at 1/1000 (1.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in parental HAP1 cell line (shown in green) and no staining in FUS KO HAP1 cells. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling TIE2 with ab307374 at 1/100 (5.0 μg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in HUVEC cells. Negative control : HEK-293T (PMID : 17189382). ab195889 was used to counterstain tubulin at 1/200 (2.5μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

ab196384 staining Cardiac Troponin I in A673 (Human muscle Ewing's Sarcoma cell line) cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196384 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MeWo(human malignant melanoma fibroblast) cells labelling ASAH1 with ab322109 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MeWo cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Negative control : NCI-H1299 (PMID : 26553872). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling Soluble TNF Receptor I + Fc Fragment with ab308506 at 1/50 (10.52 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in A549 cell line.Negative control : Raji (PMID : 10583368, PMID : 8144934). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HDLM-2 (human Hodgkin lymphoma cell) cells labeling IL-2 Receptor with ab307865 at 1/50 dilution (10.0 ug/ml) (Green). Confocal image showing cytoplasmic staining in HDLM-2 cells. Negative control : PC-3 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Green). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

ab109028 staining HP1 alpha in HeLa (human cervical adenocarcinoma epithelial cell). The cells were fixed with 100% methanol, permeabilized with 0.1% TritonX-100. The cells were then incubated with ab109028 at 1 : 100 dilution. Cells were then incubated with ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1 : 1000 dilution (shown in green). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as counterstain at 1 : 200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Confocal image showing nuclear staining in HeLa cell line.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CLDN1 KO A431(CLDN1 knockout human epidermoid carcinoma epithelial cell) cells labelling Claudin 1 with ab307692 at 1/250 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution (Green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) ( ab195889 ) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is AlexaFluor488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Confocal image showing membranous and cytoplasmic staining in wildtype A431 cells and showing no staining in CLDN1 knockout A431 cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling ASAH1 with ab322109 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SK-MEL-28 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Negative control : A375 (PMID : 26553872). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labelling PADI2 / PAD2 with ab322197 at 1/500 (1.008 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic and nuclear staining in SK-BR-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : MCF-7 (PMID : 23110523).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Fibrillarin with ab314506 at 1/500 (0.994 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nucleolar staining in HeLa cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

ab195889 staining alpha Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated with ab195889 at 1/167 dilution (shown in psedo-color red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

This product gave a positive signal in 100% methanol (10 min) fixed HeLa cells under the same testing conditions.

Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Ndufs4 with ab309701 at 1/50 (10.0 µg/ml) dilution.

Confocal image showing cytoplasmic staining in HeLa cell line (shown in green).
The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with ab305363 at 1/50 dilution (10.0 ug/ml) (Green). Confocal image showing nucleolar staining in HeLa cells.

Negative control : MCF7 (PMID : 29904067).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Ndufs4 with ab309701 at 1/50 (10.0 µg/ml) dilution.

Confocal image showing cytoplasmic staining in HepG2 cell line (shown in green).
The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Eph receptor B4/HTK with ab305102 at 1/100 (5.15 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in MCF-7 cell line.Negative control : 786-O (PMID : 34399807).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Biotin with ab324266 at 1/1000 (0.525 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing positive staining in HeLa cells (shown in green) pre-incubated with 0.5 ug/ml biotinylated Anti-Vimentin antibody [EPR3776] – Chicken IgY (Chimeric) (ab316214) at 37℃ for 1 hour (Panel A). In contrast, no positive staining is detected when the cells are incubated only with the Anti-Biotin antibody (ab324266) under the same conditions(Panel B). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblasts) cells labelling FOXL2 with primary antibody anti-FOXL2 (ab246511) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing mainly nuclear staining in K-562 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling CARD9 with ab321988 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Low expression : A549 (PMID : 32164050 ) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labeling SIGLEC5 with ab307846 at 1/50 dilution (9.72 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing membranous and cytoplasmic staining in U-937 cell line. Negative control : K-562 (PMID : 9731071). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling ESM1 with ab322145 at 1/500 (1.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : Daudi (PMID : 8702785).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Respiratory Syncytial Virus M2-1 Protein  with ab323319 at 1/4000 (0.129 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Human respiratory syncytial virus A (HRSVA) and Human respiratory syncytial virus B (HRSVB) M2-1 protein expression vectors containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling DAP12 with ab323318 at 1/1000 (0.538 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing no staining in Jurkat cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Negative control : Jurkat (PMID : 9490415).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling CXCL5 with ab305100 at 1/500 (1.012 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased cytoplasmic staining in A549 cells treated with starved overnight, then add TPA (10 nM), TNF alpha (10 ng/ml) and BSA (0.1%) for 24 h and treated with BFA (300 ng/ml) for another 3 h. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized SMARCC1 KO HeLa (SMARCC1 knockout human cervical adenocarcinoma epithelial cell) (ab264859) cells labelling SMARCC1/BAF155 with ab305037 at 1/100 (4.75 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing no staining in SMARCC1 knockout Hela cells and showing nuclear staining in wildtype Hela cells.The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8) is observed. ab195889 was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling YKL-40/CHI3L1 with ab305035 at 1/50 (10.92 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cell line.Negative control : A-204.The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Hsp70 with ab307398 at 1/50 dilution (10 ug/ml) (Green). Confocal image showing cytoplasmic and nuclear staining in HeLa cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EPHB4 KO HEK293T (ab266733) cells labelling Eph receptor B4/HTK with ab305102 at 1/100 (5.15 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in parental HEK293T cell line, but no staining in EPHB4 KO HEK293T cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling TLS/FUS with ab309787 at 1/50 (10.0 ug/ml) dilution (Green).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Confocal image showing nuclear staining in HepG2 cell line.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labeling EGFR with ab307848 at 1/50 dilution (10.0 µg/ml). Confocal image showing membranous and weak cytoplasmic staining in A431 cells. Negative control : Jurkat (PMID : 24045666). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NR3C1 KO HeLa (NR3C1 knockout human cervical adenocarcinoma epithelial cell) (ab261766) cells labelling Glucocorticoid receptor with ab305050 at 1/50 (10.24 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing no staining in NR3C1 knockout HeLa cells and showing nuclear and weak cytoplasmic staining in wildtype HeLa cells. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC

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Immunocytochemistry - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized LNCaP (human prostate carcinoma epithelial cell) cells labelling STEAP1 with ab290723 at 1/250 (1.664 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in LNCaP cell lineNegative control : MCF-7 (PMID : 10588738) is observed. ab195889 was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labeling CD90 / Thy1 with ab307736 at 1/250 dilution (1.8 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing membranous and cytoplasmic staining in U-2 OS cell line, no staining was observed in K-562 cell line. Negative control : K-562 (PMID : 7683034). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

• ICC

Supplier Data

Immunocytochemistry - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEL (human erythroleukemia erythroblast) cells labelling GPVI with ab305060 at 1/100 (4.77 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing increased membranal and cytoplasmic staining in HEL cells treated with Phorbol-12-myristate-13-acetate (10 nM) for 48 hours.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8) is observed. ab195889 was used to counterstain tubulin at 1/200 2.5 ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling Survivin with ab307852 at 1/500 dilution (1.0 ug/ml) (Green). Confocal image showing kinetochores and midbody (arrow) staining in the HeLa cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling Ubiquitin (phospho S65) with ab303542 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing increased cytoplasmic and nuclear staining in PC-3 cells treated with CCCP (30 μM) for 6 h is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labelling PADI2 / PAD2 with ab324331 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and nuclear staining in SK-BR-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : MCF-7 (PMID : 23110523).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 786-O (human kidney epithelial cell) cells labelling alpha Tubulin (tri methyl K40) with ab208330 at 1/500 (1.862 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing positive staining in 786-O cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling CD68 with ab303565 at 1/100 (4.77 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cell lineNegative control : Jurkat (PMID : 17583472) is observed. ab195889 was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195889)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-204 (human muscle rhabdomyosarcoma cell) cells labeling PDGFR alpha with ab307855 at 1/50 dilution (10.0 ug/ml) (Green). Confocal image showing membranous and cytoplasmic staining in A-204 cells. Negative control : A-172 (PMID : 8425771). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).