AB312761

重组Alexa Fluor® 568荧光Anti-Ubiquitin (linkage-specific K63)抗体[EPR8590-448]

Alexa Fluor® 568 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal UBC Linkage-specific K63 antibody - conjugated to Alexa Fluor® 568.

查看别名

Polyubiquitin-C, UBC

不同偶联物与剂型 (11)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
578 PE

PE Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
660 APC

APC Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
HRP

HRP Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
Unconjugated

Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
Carrier free

Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - Low endotoxin, Azide free
Carrier free

Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8590-448

亚型

IgG

偶联物

Alexa Fluor® 568

激发波长/发射波长

Ex: 578nm, Em: 603nm

不含载体蛋白

应用

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ubiquitin K63 only is a modification of the protein ubiquitin which involves linking ubiquitin molecules through lysine 63 (K63) to form a chain. Ubiquitin itself is a small protein with a mass of about 8.6 kDa and it can be found throughout the cell. This specific type of ubiquitin chain is not involved in tagging proteins for degradation but instead serves other signaling functions. Ubiquitin K63 only chains assemble on a variety of substrate proteins influencing their activity and interactions.

Biological function summary

The K63-linked ubiquitin chains play an important role in cellular signaling processes and protein interactions. These chains often act as docking sites for proteins involved in processes like DNA repair cell signaling and endocytosis. Ubiquitin K63 only chains also participate in forming protein complexes that aid in signal transduction. Furthermore they help modulate the activity of proteins involved in immune responses by facilitating their interaction with other signaling molecules.

Pathways

K63-linked ubiquitin chains have a big role in the NF-kappa B signaling pathway and the DNA damage response pathway. In the NF-kappa B pathway these ubiquitin chains help activate the pathway by aiding in the assembly of essential protein complexes like the IKK complex. In the DNA damage response pathway K63-linked ubiquitination assists in recruiting the appropriate repair proteins to sites of damage interacting with proteins like BRCA1 to facilitate repair mechanisms.

Abnormalities in K63-linked ubiquitination have been implicated in cancer and neurodegenerative diseases. Dysregulated K63 ubiquitin linkage can lead to improper signaling in the NF-kappa B pathway contributing to cancer progression by affecting genes related to cell growth and survival. In neurodegenerative diseases faulty K63 ubiquitination can disrupt protein homeostasis and cellular stress responses potentially involving proteins like Parkin which associates with mitochondrial quality control and Parkinson's disease pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. During ubiquitination, the acceptor ubiquitin is positioned in the active site via direct interaction with the E2 ubiquitin-conjugating enzymes such as UBE2R2 (PubMed : 38326650).

See full target information UBC Linkage-specific K63

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

重组Alexa Fluor® 568荧光Anti-Ubiquitin (linkage-specific K63)抗体[EPR8590-448]