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AB312637

重组Alexa Fluor® 568荧光Anti-CD20抗体[EP459Y]

Alexa Fluor® 568 Anti-CD20 antibody [EP459Y]

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Rabbit Recombinant Monoclonal CD20 antibody - conjugated to Alexa Fluor® 568.

查看别名

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

不同偶联物与剂型 (12)

  • Carrier free

    Anti-CD20 antibody [EP459Y] - Mouse IgG1 (Chimeric) - BSA and Azide free

  • Carrier free

    Anti-CD20 antibody [EP459Y] - Mouse IgG2a (Chimeric) - BSA and Azide free

  • Unconjugated

    Anti-CD20 antibody [EP459Y]

  • 660 APC

    APC Anti-CD20 antibody [EP459Y]

  • HRP

    HRP Anti-CD20 antibody [EP459Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD20 antibody [EP459Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD20 antibody [EP459Y]

  • Carrier free

    Anti-CD20 antibody [EP459Y] - Rat IgG2a (Chimeric) - BSA and Azide free

  • 578 PE

    PE Anti-CD20 antibody [EP459Y]

  • Carrier free

    Anti-CD20 antibody [EP459Y] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD20 antibody [EP459Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD20 antibody [EP459Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP459Y

亚型

IgG

偶联物

Alexa Fluor® 568

激发波长/发射波长

Ex: 578nm, Em: 603nm

不含载体蛋白

No

应用

Target Binding Affinity, Antibody Labelling

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

For optimal performance in IHC, the primary antibody should be incubated overnight at 4°.

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为什么推荐这个?

我们推荐这个产品,因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书,以确认其是否符合您的实验需求;若有疑问,也可联系我们的技术团队获取协助。

产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
Biological function summary

CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.

Pathways

CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.

CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1

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详情请参阅我们的条款与条件。

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