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AB312591

重组Alexa Fluor® 568荧光Anti-Calpain 1抗体[EPR3319]

Alexa Fluor® 568 Anti-Calpain 1 antibody [EPR3319]

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Rabbit Recombinant Monoclonal Calpain 1 antibody - conjugated to Alexa Fluor® 568.

查看别名

CANPL1, PIG30, CAPN1, Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, CANP 1, muCANP

不同偶联物与剂型 (10)

  • Unconjugated

    Anti-Calpain 1 antibody [EPR3319]

  • 578 PE

    PE Anti-Calpain 1 antibody [EPR3319]

  • 660 APC

    APC Anti-Calpain 1 antibody [EPR3319]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Calpain 1 antibody [EPR3319]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Calpain 1 antibody [EPR3319]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Calpain 1 antibody [EPR3319]

  • HRP

    HRP Anti-Calpain 1 antibody [EPR3319]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Calpain 1 antibody [EPR3319]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Calpain 1 antibody [EPR3319]

  • Carrier free

    Anti-Calpain 1 antibody [EPR3319] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3319

亚型

IgG

偶联物

Alexa Fluor® 568

激发波长/发射波长

Ex: 578nm, Em: 603nm

不含载体蛋白

No

应用

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody recognises the large subunit of Calpain

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为什么推荐这个?

我们推荐这个产品,因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书,以确认其是否符合您的实验需求;若有疑问,也可联系我们的技术团队获取协助。

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 1 also known as CAPN1 is a calcium-dependent cysteine protease with a mass of approximately 80 kDa. It exhibits calpain activity by cleaving substrate proteins in a regulated manner. Calpain 1 expresses in various tissues including muscle brain and lens suggesting its involvement in diverse cellular processes. The enzyme is part of a larger calpain family with calpain-1 calpain-2 and other isoforms that demonstrate differing substrate specificities and regulatory mechanisms.
Biological function summary

Calpain 1 participates in cellular functions such as signal transduction cell cycle progression and apoptosis. It is not part of a complex but operates independently to modulate cellular responses by processing different target proteins. Calpain protein activity impacts cytoskeletal remodeling and membrane repair important processes in cell mobility and stability. Through its proteolytic actions calpain 1 influences neural plasticity and muscle function demonstrating its broad biological significance.

Pathways

Calpain 1 functions within the calcium signaling and apoptotic pathways highlighting its role in regulating cellular survival and death. Calcium influx triggers calpain activity linking it to proteins such as calmodulin and calcineurin that also respond to calcium signals. Calpain-1 modulates apoptosis by targeting Bcl-2 family proteins influencing cell fate during stress responses. The calpain assay measures its activity providing insights into its contribution to these vital pathways.

Calpain 1 connects to neurodegenerative diseases like Alzheimer's and muscular diseases such as muscular dystrophy. Alzheimer's disease features abnormal calpain activity affecting tau protein and amyloid precursor protein processing. In muscular dystrophy calpain-1's unregulated activity leads to excessive muscle degradation aggravating the disease condition. Its interaction with spectrin and dystrophin proteins suggests a mechanistic role in the pathogenesis of these disorders pinpointing calpain-1 as a potential therapeutic target.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed : 19617626, PubMed : 21531719, PubMed : 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed : 23707407). Cleaves and activates caspase-7 (CASP7) (PubMed : 19617626).
See full target information CAPN1

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详情请参阅我们的条款与条件。

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Alternative Version
Primary Antibodies

AB239930

Anti-Calpain 1 antibody [EPR3319] - BSA and Azide free

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